Insect Genomics
Methods and Protocols
Editors: Brown, Susan, Pfrender, Michael (Eds.)
Free Preview- Includes cutting-edge techniques for the study of insect genomics
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.
- Table of contents (15 chapters)
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Arthropod Genome Sequencing and Assembly Strategies
Pages 1-14
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Genome Size Estimation and Quantitative Cytogenetics in Insects
Pages 15-26
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Isolation of High Molecular Weight DNA from Insects
Pages 27-32
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Long Range Sequencing and Validation of Insect Genome Assemblies
Pages 33-44
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Integrated Modeling of Structural Genes Using MCuNovo
Pages 45-57
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Insect Genomics
- Book Subtitle
- Methods and Protocols
- Editors
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- Susan Brown
- Michael Pfrender
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1858
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8775-7
- DOI
- 10.1007/978-1-4939-8775-7
- Hardcover ISBN
- 978-1-4939-8774-0
- Softcover ISBN
- 978-1-4939-9389-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 237
- Number of Illustrations
- 11 b/w illustrations, 23 illustrations in colour
- Topics
