- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.
- Table of contents (26 chapters)
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Molecular Dynamics Simulations of the SNARE Complex
Pages 3-13
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Mesoscale Computational Modeling of Protein-Membrane Interactions Based on Continuum Mean-Field Theory
Pages 15-31
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EPR Lineshape Analysis to Investigate the SNARE Folding Intermediates
Pages 33-51
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Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion
Pages 53-69
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SNAREpin Assembly: Kinetic and Thermodynamic Approaches
Pages 71-93
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- SNAREs
- Book Subtitle
- Methods and Protocols
- Editors
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- Rutilio Fratti
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1860
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8760-3
- DOI
- 10.1007/978-1-4939-8760-3
- Hardcover ISBN
- 978-1-4939-8759-7
- Softcover ISBN
- 978-1-4939-9385-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 407
- Number of Illustrations
- 8 b/w illustrations, 66 illustrations in colour
- Topics
