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  • © 2018

The CRAC Channel

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1843)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells

    • Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald L. Gill, Youjun Wang
    Pages 1-16
  3. Mn2+ Quenching Assay for Store-Operated Calcium Entry

    • Zui Pan, Sangyong Choi, Yanhong Luo
    Pages 55-62
  4. Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations

    • Pedro C. Redondo, Alejandro Berna-Erro, Natalia Dionisio, Juan A. Rosado
    Pages 69-82
  5. CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR

    • Charlotte Dubois, Vyacheslav Lehenkyi, Natalia Prevarskaya
    Pages 95-106
  6. Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures

    • Sumita Chakraborty, Gaiti Hasan
    Pages 125-136
  7. Study of the Endogenous CRAC Channel Using shRNA-Mediated Gene Silencing

    • Xuexin Zhang, Amy M. Spinelli, Wei Zhang, Mohamed Trebak
    Pages 137-145
  8. Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel

    • Guolin Ma, Lian He, Ji Jing, Peng Tan, Yun Huang, Yubin Zhou
    Pages 147-166
  9. High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy

    • Andras T. Deak, Benjamin Gottschalk, Emrah Eroglu, Rene Rost, Markus Waldeck-Weiermair, Wolfgang F. Graier et al.
    Pages 175-187
  10. Single-Channel Single-Molecule Detection (SC-SMD) System

    • Laura G. Ceballos, Alexander Asanov, Luis Vaca
    Pages 189-201
  11. Back Matter

    Pages 203-204

About this book

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.


Editors and Affiliations

  • IRSET Inserm UMR 1085/ UR1, Rennes, France

    Aubin Penna

  • CNRS ERL 7003, Laboratoire STIM, Poitiers, France

    Bruno Constantin

Bibliographic Information

  • Book Title: The CRAC Channel

  • Book Subtitle: Methods and Protocols

  • Editors: Aubin Penna, Bruno Constantin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8704-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8702-3Published: 10 September 2018

  • Softcover ISBN: 978-1-4939-9370-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8704-7Published: 10 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 204

  • Number of Illustrations: 15 b/w illustrations, 26 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access