Lipases and Phospholipases
Methods and Protocols
Editors: SANDOVAL, GEORGINA (Ed.)
Free Preview- Includes cutting-edge methods and protocols for the study of lipases and phospholipases
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
- Table of contents (22 chapters)
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Lipases: An Overview
Pages 3-38
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Carbohydrate Esterases: An Overview
Pages 39-68
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Phospholipases: An Overview
Pages 69-105
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Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates
Pages 109-117
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A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates
Pages 119-128
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Table of contents (22 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Lipases and Phospholipases
- Book Subtitle
- Methods and Protocols
- Editors
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- GEORGINA SANDOVAL
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1835
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8672-9
- DOI
- 10.1007/978-1-4939-8672-9
- Hardcover ISBN
- 978-1-4939-8671-2
- Softcover ISBN
- 978-1-4939-9361-1
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIV, 437
- Number of Illustrations
- 32 b/w illustrations, 57 illustrations in colour
- Topics