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Lipases and Phospholipases

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Georgina Sandoval

    1. CIATEJ, Guadalajara, Mexico

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  • Includes cutting-edge methods and protocols for the study of lipases and phospholipases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1835)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Overview of Lipases, Phospholipases and Esterases

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Lipases: An Overview

      • Leticia Casas-Godoy, Francisco Gasteazoro, Sophie Duquesne, Florence Bordes, Alain Marty, Georgina Sandoval
      Pages 3-38

    3. Carbohydrate Esterases: An Overview

      • Mariana Armendáriz-Ruiz, Jorge Alberto Rodríguez-González, Rosa María Camacho-Ruíz, Juan Carlos Mateos-Díaz
      Pages 39-68

    4. Phospholipases: An Overview

      • Ahmed Aloulou, Renaud Rahier, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham
      Pages 69-105

  3. Activity Screening and Expression

    1. Front Matter

      Pages 107-107
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    2. Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates

      • Dolores Reyes-Duarte, Cristina Coscolín, Mónica Martínez-Martínez, Manuel Ferrer, Humberto García-Arellano
      Pages 109-117

    3. A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities Using α-Eleostearic Acid-Containing Substrates

      • Meddy El Alaoui, Laurent Soulère, Alexandre Noiriel, Priscila Sutto-Ortiz, Lucie Grand, Florence Popowycz et al.
      Pages 119-128

    4. Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline

      • Renaud Rahier, Houda Abla, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham
      Pages 129-138

    5. Lipase and Phospholipase Activity Methods for Marine Organisms

      • H. Nolasco-Soria, F. Moyano-López, F. Vega-Villasante, Alberto del Monte-Martínez, D. Espinosa-Chaurand, E. Gisbert et al.
      Pages 139-167

    6. Functional Expression of Plant Lipases: The Case of CpLip1 from Carica papaya

      • Ivanna Rivera, Abel Gutiérrez-Ortega, Georgina Sandoval
      Pages 169-178

    7. Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2

      • Nabil Smichi, Neila Achouri, Alexandre Noiriel, Vincent Arondel, Abdelkarim Abousalham, Youssef Gargouri et al.
      Pages 179-189

    8. Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies

      • Yani Arhab, Renaud Rahier, Alexandre Noiriel, Mickael V. Cherrier, Abdelkarim Abousalham
      Pages 191-201

  4. Optimization of the Biocatalyst Production and Performances

    1. Front Matter

      Pages 203-203
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    2. Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review

      • Francisco Valero
      Pages 205-216

    3. Solid-State Fermentation as an Economic Production Method of Lipases

      • Doddy Denise Ojeda-Hernández, Ricardo Cosío-Cuadros, Georgina Sandoval, Jorge Alberto Rodríguez-González, Juan Carlos Mateos-Díaz
      Pages 217-228

    4. Synthetic Biology to Improve the Production of Lipases and Esterases (Review)

      • Heber Gamboa-Melendez, Macarena Larroude, Young Kyoung Park, Pauline Trebul, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro
      Pages 229-242

    5. Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases

      • Alberto del Monte-Martínez, Bessy V. Cutiño-Avila, Jorge González-Bacerio
      Pages 243-283

  5. Applications of Lipases, Phospholipases and Esterases

    1. Front Matter

      Pages 285-285
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    2. Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides

      • Jose L. Gonzalez-Alfonso, Leticia Casas-Godoy, Javier Arrizon, Daniel Arrieta-Baez, Antonio O. Ballesteros, Georgina Sandoval et al.
      Pages 287-296
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Keywords

About this book

This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.

Editors and Affiliations

  • CIATEJ, Guadalajara, Mexico

    Georgina Sandoval

Bibliographic Information

  • Book Title: Lipases and Phospholipases

  • Book Subtitle: Methods and Protocols

  • Editors: Georgina Sandoval

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8672-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8671-2Published: 15 August 2018

  • Softcover ISBN: 978-1-4939-9361-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8672-9Published: 14 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 437

  • Number of Illustrations: 32 b/w illustrations, 57 illustrations in colour

  • Topics: Biochemistry, general

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