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Plant Transcription Factors

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of plant transcription factors
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1830)

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Hardcover Book USD 219.99
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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. Preparation of Plant Materials

    1. Front Matter

      Pages 59-59
    2. Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs

      • Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, Utpal Nath
      Pages 61-79
    3. A Specific Knockdown of Transcription Factor Activities in Arabidopsis

      • Beibei Zheng, Bennett Thomson, Frank Wellmer
      Pages 81-92
    4. Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis

      • Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
      Pages 93-108
    5. Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines

      • Tatyana Radoeva, Shunsuke Saiga, Dolf Weijers
      Pages 127-139
    6. Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells

      • Kyohei Uemoto, Takashi Araki, Motomu Endo
      Pages 141-148
  3. Detection of Expression Levels

    1. Front Matter

      Pages 161-161
    2. Genome-Wide TSS Identification in Maize

      • María Katherine Mejia-Guerra, Wei Li, Andrea I. Doseff, Erich Grotewold
      Pages 239-256

About this book

This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.

Editors and Affiliations

  • Plant Stem Cell Regulation and Floral Patterning Laboratory, Nara Institute of Science and Technology, Ikoma, Japan

    Nobutoshi Yamaguchi

Bibliographic Information

  • Book Title: Plant Transcription Factors

  • Book Subtitle: Methods and Protocols

  • Editors: Nobutoshi Yamaguchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8657-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8656-9Published: 25 July 2018

  • Softcover ISBN: 978-1-4939-9356-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8657-6Published: 24 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 396

  • Number of Illustrations: 23 b/w illustrations, 58 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access