- Includes cutting-edge methods and protocols for the study of two-hybrid systems
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.
- Table of contents (24 chapters)
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Protein Interactomics by Two-Hybrid Methods
Pages 1-14
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Making the Right Choice: Critical Parameters of the Y2H Systems
Pages 17-28
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Identification of Protein–Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening
Pages 29-48
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Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System
Pages 49-60
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The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions
Pages 61-73
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Table of contents (24 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Two-Hybrid Systems
- Book Subtitle
- Methods and Protocols
- Editors
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- Luis Oñate-Sánchez
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1794
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-7871-7
- DOI
- 10.1007/978-1-4939-7871-7
- Hardcover ISBN
- 978-1-4939-7870-0
- Softcover ISBN
- 978-1-4939-9310-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 355
- Number of Illustrations
- 8 b/w illustrations, 50 illustrations in colour
- Topics
*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works are not included.
