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Methods in Molecular Biology

Tissue Proteomics

Methods and Protocols

Editors: Sarwal, Minnie, Sigdel, Tara (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 142,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7854-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7852-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from native tissues, fractionation strategies for protein analysis, and GeLC-MS as a sample preparation method for sample preparation for proteomics using minimal amount of tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Table of contents (24 chapters)

  • Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics

    Treumann, Achim (et al.)

    Pages 1-9

  • Discovery of Immune Reactive Human Proteins by High-Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA

    Sigdel, Tara K. (et al.)

    Pages 11-21

  • HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells

    Valenzuela, Nicole (et al.)

    Pages 23-41

  • Characterization of Protein Complexes Using Chemical Cross-Linking Coupled Electrospray Mass Spectrometry

    Cummins, Timothy D. (et al.)

    Pages 43-61

  • Optimization for Peptide Sample Preparation for Urine Peptidomics

    Sigdel, Tara K. (et al.)

    Pages 63-72

    Preview Buy Chapter 48,34 €

Buy this book

eBook 142,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7854-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7852-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Tissue Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Minnie Sarwal
  • Tara Sigdel
Series Title
Methods in Molecular Biology
Series Volume
1788
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7854-0
DOI
10.1007/978-1-4939-7854-0
Hardcover ISBN
978-1-4939-7852-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 320
Number of Illustrations and Tables
6 b/w illustrations, 44 illustrations in colour
Topics