- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.
The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com. - About the authors
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- Table of contents (24 chapters)
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Culture of Human Monocyte-Derived Macrophages
Pages 1-11
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A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages
Pages 13-28
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Growing Murine Bone Marrow-Derived Macrophages
Pages 29-33
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Immortalization of Murine Bone Marrow-Derived Macrophages
Pages 35-49
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Isolation and Identification of Murine Serous Cavity Macrophages
Pages 51-67
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Table of contents (24 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Macrophages
- Book Subtitle
- Methods and Protocols
- Editors
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- Germain Rousselet
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1784
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-7837-3
- DOI
- 10.1007/978-1-4939-7837-3
- Hardcover ISBN
- 978-1-4939-7836-6
- Softcover ISBN
- 978-1-4939-9300-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 278
- Number of Illustrations
- 8 b/w illustrations, 35 illustrations in colour
- Topics
