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Root Development

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Daniela Ristova

    1. Gregor Mendel Institute of Molecular Plant Biology, Vienna, Austria

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    You can also search for this editor in PubMed   Google Scholar

  2. Elke Barbez

    1. Institute of Applied Genetics & Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of plant root biology and development
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1761)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
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  2. Genetic and Phenotypic Analyses

    1. Front Matter

      Pages 1-1
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    2. Measuring Plant Root Traits Under Controlled and Field Conditions: Step-by-Step Procedures

      • Benjamin M. Delory, Emanuela W. A. Weidlich, Richard van Duijnen, Loïc Pagès, Vicky M. Temperton
      Pages 3-22

    3. Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean

      • Larry M. York
      Pages 23-32

    4. Developmental Analysis of Arabidopsis Root Meristem

      • Riccardo Di Mambro, Sabrina Sabatini
      Pages 33-45

    5. Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana

      • Selene Napsucialy-Mendivil, Joseph G. Dubrovsky
      Pages 47-75

    6. Adapting the Lateral Root-Inducible System to Medicago truncatula

      • Violaine Herrbach, Fabienne Maillet, Sandra Bensmihen
      Pages 77-83

    7. Characterization of Root Epidermal Cell Patterning and Differentiation in Arabidopsis

      • Jorge E. Salazar-Henao, Girish Mokkapati, Eunice Han Xian Khor, Yi-Chia Chou, Wann-Neng Jane, Wolfgang Schmidt
      Pages 85-93

    8. In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls

      • Hoang Khai Trinh, Inge Verstraeten, Danny Geelen
      Pages 95-102

    9. Root Gravitropism: Quantification, Challenges, and Solutions

      • Lukas Muller, Malcolm J. Bennett, Andy French, Darren M. Wells, Ranjan Swarup
      Pages 103-112

  3. Microscopy and Cell Biology

    1. Front Matter

      Pages 113-113
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    2. Calcium Ion Dynamics in Roots: Imaging and Analysis

      • Ellie Himschoot, Melanie Krebs, Alex Costa, Tom Beeckman, Steffen Vanneste
      Pages 115-130

    3. Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia

      • Michael Karampelias, Ricardo Tejos, Jiří Friml, Steffen Vanneste
      Pages 131-143

    4. Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development

      • Paolo Baesso, Ricardo S. Randall, Giovanni Sena
      Pages 145-163

    5. Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting

      • Beatriz Lagunas, Liam Walker, Rana Muhammad Fraz Hussain, Ian Hands-Portman, Kate Woolley-Allen, Miriam L. Gifford
      Pages 165-175

    6. Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots

      • Wei Xuan, Davy Opdenacker, Steffen Vanneste, Tom Beeckman
      Pages 177-190

    7. Cortical Cell Length Analysis During Gravitropic Root Growth

      • Maria Schöller, Jürgen Kleine-Vehn, Elena Feraru
      Pages 191-197

    8. Growth Rate Normalization Method to Assess Gravitropic Root Growth

      • Maria Schöller, Elizabeth Sarkel, Jürgen Kleine-Vehn, Elena Feraru
      Pages 199-208

    9. Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

      • Sascha Waidmann, Lucinda De-Araujo, Jürgen Kleine-Vehn, Barbara Korbei
      Pages 209-220

  4. Genome-Wide Methods and Systems Biology

    1. Front Matter

      Pages 221-221
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Keywords

About this book

This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.

Editors and Affiliations

  • Gregor Mendel Institute of Molecular Plant Biology, Vienna, Austria

    Daniela Ristova

  • Institute of Applied Genetics & Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

    Elke Barbez

Bibliographic Information

  • Book Title: Root Development

  • Book Subtitle: Methods and Protocols

  • Editors: Daniela Ristova, Elke Barbez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7747-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7746-8Published: 11 March 2018

  • Softcover ISBN: 978-1-4939-9273-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7747-5Published: 10 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 324

  • Number of Illustrations: 18 b/w illustrations, 65 illustrations in colour

  • Topics: Plant Sciences

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