Methods in Molecular Biology

Chaperones

Methods and Protocols

Editors: Calderwood, Stuart K., Prince, Thomas L. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook 130,89 €
price for Spain (gross)
  • ISBN 978-1-4939-7477-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-7476-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.  Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (29 chapters)

  • Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

    Jin, Xiongjie (et al.)

    Pages 1-22

  • Role of Heat Shock Factors in Stress-Induced Transcription

    Murshid, Ayesha (et al.)

    Pages 23-34

  • Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter

    Kijima, Toshiki (et al.)

    Pages 35-45

  • Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay

    Taipale, Mikko

    Pages 47-58

  • Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay

    Waarde-Verhagen, Maria A. W. H. (et al.)

    Pages 59-74

    Preview Buy Chapter 48,34 €

Buy this book

eBook 130,89 €
price for Spain (gross)
  • ISBN 978-1-4939-7477-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-7476-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Chaperones
Book Subtitle
Methods and Protocols
Editors
  • Stuart K. Calderwood
  • Thomas L. Prince
Series Title
Methods in Molecular Biology
Series Volume
1709
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7477-1
DOI
10.1007/978-1-4939-7477-1
Hardcover ISBN
978-1-4939-7476-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIX, 445
Number of Illustrations and Tables
33 b/w illustrations, 36 illustrations in colour
Topics