Chaperones
Methods and Protocols
Editors: Calderwood, Stuart K., Prince, Thomas L. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- Table of contents (29 chapters)
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Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
Pages 1-22
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Role of Heat Shock Factors in Stress-Induced Transcription
Pages 23-34
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Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Pages 35-45
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Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
Pages 47-58
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Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay
Pages 59-74
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Table of contents (29 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Chaperones
- Book Subtitle
- Methods and Protocols
- Editors
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- Stuart K. Calderwood
- Thomas L. Prince
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1709
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7477-1
- DOI
- 10.1007/978-1-4939-7477-1
- Hardcover ISBN
- 978-1-4939-7476-4
- Softcover ISBN
- 978-1-4939-8498-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIX, 445
- Number of Illustrations
- 33 b/w illustrations, 36 illustrations in colour
- Topics
