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Phage Display

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Michael Hust

    1. Technische Universität Braunschweig, Braunschweig, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Theam Soon Lim

    1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1701)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Construction of Antibody Phage Display Libraries

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Construction of Human Immune and Naive scFv Libraries

      • Jonas Kügler, Florian Tomszak, André Frenzel, Michael Hust
      Pages 3-24

    3. Construction of Naive and Immune Human Fab Phage-Display Library

      • Noorsharmimi Omar, Theam Soon Lim
      Pages 25-44

    4. Construction of Synthetic Antibody Phage-Display Libraries

      • Johan Nilvebrant, Sachdev S. Sidhu
      Pages 45-60

    5. Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes

      • Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, Bin Liu
      Pages 61-82

    6. Construction of Macaque Immune-Libraries

      • Arnaud Avril, Sebastian Miethe, Michael Hust, Thibaut Pelat
      Pages 83-112

    7. Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format

      • Ulrike S. Diesterbeck
      Pages 113-131

    8. Construction of Rabbit Immune Antibody Libraries

      • Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim
      Pages 133-146

    9. Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries

      • Julius Grzeschik, Doreen Könning, Steffen C. Hinz, Simon Krah, Christian Schröter, Martin Empting et al.
      Pages 147-167

    10. Construction of High-Quality Camel Immune Antibody Libraries

      • Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, Baptiste Monsion
      Pages 169-187

    11. Construction of Chicken Antibody Libraries

      • Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt
      Pages 189-203

    12. Construction and Selection of Affilin® Phage Display Libraries

      • Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, Eva Bosse-Doenecke
      Pages 205-238

    13. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies

      • Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau
      Pages 239-253

    14. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach

      • Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung
      Pages 255-269

  3. Selection Strategies for Antibodies

    1. Front Matter

      Pages 271-271
      Download chapter PDF

    2. Parallelized Antibody Selection in Microtiter Plates

      • Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel et al.
      Pages 273-284

    3. Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning

      • Chai Fung Chin, Yee Siew Choong, Theam Soon Lim
      Pages 285-299

    4. Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection

      • Angela Chiew Wen Ch’ng, Nurul Hamizah Binti Hamidon, Zoltán Konthur, Theam Soon Lim
      Pages 301-319

    5. Phage Display and Selections on Cells

      • Wieland Fahr, André Frenzel
      Pages 321-330
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Keywords

About this book

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.

This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

Editors and Affiliations

  • Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  • Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

Bibliographic Information

  • Book Title: Phage Display

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Hust, Theam Soon Lim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7447-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7446-7Published: 21 November 2017

  • Softcover ISBN: 978-1-4939-8488-6Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7447-4Published: 08 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 562

  • Number of Illustrations: 17 b/w illustrations, 43 illustrations in colour

  • Topics: Immunology

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