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Cancer Stem Cells

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Gianpaolo Papaccio

    1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

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  2. Vincenzo Desiderio

    1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of cancer stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1692)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction to Cancer Stem Cells: Past, Present, and Future

    • David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle et al.
    Pages 1-16

  3. Surface Markers for the Identification of Cancer Stem Cells

    • Vinod Gopalan, Farhadul Islam, Alfred King-yin Lam
    Pages 17-29

  4. The Role of CD44 and Cancer Stem Cells

    • Liang Wang, Xiangsheng Zuo, Keping Xie, Daoyan Wei
    Pages 31-42

  5. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay

    • Luigi Mele, Davide Liccardo, Virginia Tirino
    Pages 43-48

  6. Isolation of Cancer Stem Cells by Side Population Method

    • Masayuki Shimoda, Masahide Ota, Yasunori Okada
    Pages 49-59

  7. Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres

    • Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, Geeta Mehta
    Pages 61-75

  8. In Vitro Tumorigenic Assay: The Tumor Spheres Assay

    • Hui Wang, Anna M. Paczulla, Martina Konantz, Claudia Lengerke
    Pages 77-87

  9. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells

    • Vijayalakshmi Rajendran, Mayur Vilas Jain
    Pages 89-95

  10. Xenograft as In Vivo Experimental Model

    • Manuela Porru, Luca Pompili, Carla Caruso, Carlo Leonetti
    Pages 97-105

  11. How to Assess Drug Resistance in Cancer Stem Cells

    • Maria Laura De Angelis, Ruggero De Maria, Marta Baiocchi
    Pages 107-115

  12. Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells

    • Meenakshi Upreti
    Pages 117-128

  13. Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)

    • Anna Di Biase, Amanda K. Miles, Tarik Regad
    Pages 129-138

  14. Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

    • Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad
    Pages 139-148

  15. Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform

    • Hiromichi Kawasaki, Angela Lombardi, Michele Caraglia
    Pages 149-156

  16. Assessing DNA Methylation in Cancer Stem Cells

    • Sudipto Das, Bruce Moran, Antoinette S. Perry
    Pages 157-178

  17. Histones Acetylation and Cancer Stem Cells (CSCs)

    • Vivian Petersen Wagner, Manoela Domingues Martins, Rogerio Moraes Castilho
    Pages 179-193

  18. Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods

    • Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, Renato Franco
    Pages 195-211

  19. Circulating Tumor Cells

    • Sebastián A. García, Jürgen Weitz, Sebastian Schölch
    Pages 213-219

  20. Erratum to: Introduction to Cancer Stem Cells: Past, Present, and Future

    • David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle et al.
    Pages E1-E1
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Keywords

About this book

This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls. 

Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. 

Editors and Affiliations

  • Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio, Vincenzo Desiderio

Bibliographic Information

  • Book Title: Cancer Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Gianpaolo Papaccio, Vincenzo Desiderio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7401-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7400-9Published: 07 October 2017

  • Softcover ISBN: 978-1-4939-8474-9Published: 17 May 2018

  • eBook ISBN: 978-1-4939-7401-6Published: 05 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 223

  • Number of Illustrations: 17 b/w illustrations, 13 illustrations in colour

  • Topics: Cancer Research

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