Argonaute Proteins
Methods and Protocols
Editors: Okamura, Katsutomo, Nakanishi, Kotaro (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.
- Table of contents (16 chapters)
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Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing
Pages 1-28
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Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR
Pages 29-40
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Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
Pages 41-63
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Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
Pages 65-73
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MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
Pages 75-86
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Table of contents (16 chapters)
- Download Preface 1 PDF (40.5 KB)
- Download Sample pages 1 PDF (199.4 KB)
- Download Table of contents PDF (32.9 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Argonaute Proteins
- Book Subtitle
- Methods and Protocols
- Editors
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- Katsutomo Okamura
- Kotaro Nakanishi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1680
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7339-2
- DOI
- 10.1007/978-1-4939-7339-2
- Hardcover ISBN
- 978-1-4939-7338-5
- Softcover ISBN
- 978-1-4939-8457-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 260
- Number of Illustrations
- 11 b/w illustrations, 44 illustrations in colour
- Topics
