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Single Molecule Analysis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Erwin J. G. Peterman

    1. Department of Physics & Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1665)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Optical Tweezers

    1. Front Matter

      Pages 1-1
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    2. Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions

      • Joost van Mameren, Gijs J. L. Wuite, Iddo Heller
      Pages 3-23

    3. RNA Unzipping and Force Measurements with a Dual Optical Trap

      • Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, Ulrich Bockelmann
      Pages 25-41

    4. Protein Tethering for Folding Studies

      • Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, Sander J. Tans
      Pages 43-51

    5. Combining Structure–Function and Single-Molecule Studies on Cytoplasmic Dynein

      • Lu Rao, Maren Hülsemann, Arne Gennerich
      Pages 53-89

  3. Single-Molecule Fluorescence Microscopy

    1. Front Matter

      Pages 91-91
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    2. A Brief Introduction to Single-Molecule Fluorescence Methods

      • Siet M. J. L. van den Wildenberg, Bram Prevo, Erwin J. G. Peterman
      Pages 93-113

    3. Fluorescent Labeling of Proteins

      • Mauro Modesti
      Pages 115-134

    4. Single-Molecule Imaging of Escherichia coli Transmembrane Proteins

      • Aravindan Varadarajan, Felix Oswald, Yves J. M. Bollen
      Pages 135-144

    5. Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans

      • Jaap van Krugten, Erwin J. G. Peterman
      Pages 145-154

    6. Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy

      • Roderick P. Tas, Trusanne G. A. A. Bos, Lukas C. Kapitein
      Pages 155-171

    7. Fluorescence Microscopy of Nanochannel-Confined DNA

      • Fredrik Westerlund, Fredrik Persson, Joachim Fritzsche, Jason P. Beech, Jonas O. Tegenfeldt
      Pages 173-198

    8. Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin–Microtubule Complexes

      • Matthieu P. M. H. Benoit, Hernando Sosa
      Pages 199-216

    9. Single Molecule FRET Analysis of DNA Binding Proteins

      • Kathy R. Chaurasiya, Remus T. Dame
      Pages 217-239

  4. Atomic Force Microscopy and Other Force Methods

    1. Front Matter

      Pages 241-241
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    2. Atomic Force Microscopy: An Introduction

      • Melissa C. Piontek, Wouter H. Roos
      Pages 243-258

    3. Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy

      • MaÅ‚gorzata Grosbart, Dejan Ristić, Humberto Sánchez, Claire Wyman
      Pages 259-280

    4. Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond

      • Natalia Martín-González, Alvaro Ortega-Esteban, F. Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Pedro J. de Pablo
      Pages 281-296

    5. Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization

      • Yeonee Seol, Keir C. Neuman
      Pages 297-316
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Keywords

About this book

The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule. 

Editors and Affiliations

  • Department of Physics & Astronomy, Vrije Universiteit, Amsterdam, The Netherlands

    Erwin J. G. Peterman

Bibliographic Information

  • Book Title: Single Molecule Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Erwin J. G. Peterman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7271-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7270-8Published: 23 September 2017

  • Softcover ISBN: 978-1-4939-8437-4Published: 18 August 2018

  • eBook ISBN: 978-1-4939-7271-5Published: 21 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 369

  • Number of Illustrations: 42 b/w illustrations, 46 illustrations in colour

  • Topics: Biochemistry, general

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