Methods in Molecular Biology

Membrane Protein Structure and Function Characterization

Methods and Protocols

Editors: Lacapere, Jean-Jacques (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins.  Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2

    Senicourt, Lucile (et al.)

    Pages 1-25

  • Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex

    Souabni, Hajer (et al.)

    Pages 27-43

  • Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts

    Hartmann, Lucie (et al.)

    Pages 45-56

  • Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits

    Larrieu, Isabelle (et al.)

    Pages 57-90

  • Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins

    Fogeron, Marie-Laure (et al.)

    Pages 91-108

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-1-4939-7151-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-4939-7149-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-4939-8399-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Protein Structure and Function Characterization
Book Subtitle
Methods and Protocols
Editors
  • Jean-Jacques Lacapere
Series Title
Methods in Molecular Biology
Series Volume
1635
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7151-0
DOI
10.1007/978-1-4939-7151-0
Hardcover ISBN
978-1-4939-7149-7
Softcover ISBN
978-1-4939-8399-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 419
Number of Illustrations
29 b/w illustrations, 13 illustrations in colour
Topics