Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1632)
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Table of contents (23 protocols)
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Front Matter
About this book
This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.
Editors and Affiliations
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Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, USA
Eckart Bindewald
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RNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, USA
Bruce A. Shapiro
Bibliographic Information
Book Title: RNA Nanostructures
Book Subtitle: Methods and Protocols
Editors: Eckart Bindewald, Bruce A. Shapiro
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7138-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7137-4Published: 21 July 2017
Softcover ISBN: 978-1-4939-8395-7Published: 12 May 2018
eBook ISBN: 978-1-4939-7138-1Published: 20 July 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 383
Number of Illustrations: 20 b/w illustrations, 75 illustrations in colour
Topics: Human Genetics