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  • © 2017

Genome Editing in Animals

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1630)

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eBook USD 119.00
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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Construction and Evaluation of Zinc Finger Nucleases

    • Hiroshi Ochiai, Takashi Yamamoto
    Pages 1-24
  3. Current Overview of TALEN Construction Systems

    • Tetsushi Sakuma, Takashi Yamamoto
    Pages 25-36
  4. CRISPR/Cas9

    • Izuho Hatada, Takuro Horii
    Pages 37-42
  5. Genome Editing of Mouse by Cytoplasmic Injection

    • Takuro Horii, Izuho Hatada
    Pages 55-66
  6. Genome Editing of Rat

    • Takehito Kaneko
    Pages 101-108
  7. Genome Editing of Pig

    • Masahito Watanabe, Hiroshi Nagashima
    Pages 121-139
  8. Genome Editing of Monkey

    • Zhen Liu, Yijun Cai, Qiang Sun
    Pages 141-152
  9. Genome Editing Mediated by Primordial Germ Cell in Chicken

    • Jae Yong Han, Hong Jo Lee
    Pages 153-163
  10. Genome Editing of Medaka

    • Satoshi Ansai, Masato Kinoshita
    Pages 175-188
  11. Genome Editing of Silkworms

    • Takuya Tsubota, Hideki Sezutsu
    Pages 205-218
  12. Genome Editing in the Cricket, Gryllus bimaculatus

    • Takahito Watanabe, Sumihare Noji, Taro Mito
    Pages 219-233
  13. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease

    • Yasunori Sasakura, Keita Yoshida, Nicholas Treen
    Pages 235-245

About this book

This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan

    Izuho Hatada

Bibliographic Information

  • Book Title: Genome Editing in Animals

  • Book Subtitle: Methods and Protocols

  • Editors: Izuho Hatada

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7128-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7127-5Published: 23 June 2017

  • Softcover ISBN: 978-1-4939-8393-3Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7128-2Published: 22 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 256

  • Number of Illustrations: 22 b/w illustrations, 45 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access