- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.
- Table of contents (18 chapters)
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In Silico PCR Tools for a Fast Primer, Probe, and Advanced Searching
Pages 1-31
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Introduction on Using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis
Pages 33-64
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Long Fragment Polymerase Chain Reaction
Pages 65-74
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Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR
Pages 75-85
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Inverse PCR for Point Mutation Introduction
Pages 87-100
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Table of contents (18 chapters)
- Download Preface 1 PDF (87.1 KB)
- Download Sample pages 1 PDF (2.2 MB)
- Download Table of contents PDF (1.4 MB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- PCR
- Book Subtitle
- Methods and Protocols
- Editors
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- Lucilia Domingues
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1620
- Copyright
- 2017
- Publisher
- Springer-Verlag New York
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7060-5
- DOI
- 10.1007/978-1-4939-7060-5
- Hardcover ISBN
- 978-1-4939-7059-9
- Softcover ISBN
- 978-1-4939-8381-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 282
- Number of Illustrations
- 27 b/w illustrations, 25 illustrations in colour
- Topics
