Methods in Molecular Biology

Serum/Plasma Proteomics

Methods and Protocols

Editors: Greening, David W, Simpson, Richard (Eds.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.

Table of contents (35 chapters)

Table of contents (35 chapters)
  • Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation

    Greco, Viviana (et al.)

    Pages 3-21

  • A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Studies

    Sparrow, Rosemary L., Ph.D. (et al.)

    Pages 23-30

  • Preparation of Platelet Concentrates for Research and Transfusion Purposes

    Greening, David W., Ph.D. (et al.)

    Pages 31-42

  • Bead-Based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture

    Birgersson, Elin (et al.)

    Pages 45-54

  • Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma

    O’Grady, John P. (et al.)

    Pages 55-61

Buy this book

eBook 91,62 €
price for Spain (gross)
  • ISBN 978-1-4939-7057-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-7056-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 114,39 €
price for Spain (gross)
  • ISBN 978-1-4939-8380-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Serum/Plasma Proteomics
Book Subtitle
Methods and Protocols
Editors
  • David W Greening
  • Richard Simpson
Series Title
Methods in Molecular Biology
Series Volume
1619
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7057-5
DOI
10.1007/978-1-4939-7057-5
Hardcover ISBN
978-1-4939-7056-8
Softcover ISBN
978-1-4939-8380-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 551
Number of Illustrations
24 b/w illustrations, 70 illustrations in colour
Topics