Bacterial Protein Secretion Systems
Methods and Protocols
Editors: Journet, Laure, Cascales, Eric (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
- Table of contents (38 chapters)
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Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder
Pages 1-21
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Protein Sorting Prediction
Pages 23-57
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Cell Fractionation
Pages 59-64
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Defining Lipoprotein Localisation by Fluorescence Microscopy
Pages 65-74
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Identification of Lipoproteins Using Globomycin and Radioactive Palmitate
Pages 75-80
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Table of contents (38 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Bacterial Protein Secretion Systems
- Book Subtitle
- Methods and Protocols
- Editors
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- Laure Journet
- Eric Cascales
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1615
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7033-9
- DOI
- 10.1007/978-1-4939-7033-9
- Hardcover ISBN
- 978-1-4939-7031-5
- Softcover ISBN
- 978-1-4939-8374-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 528
- Number of Illustrations
- 26 b/w illustrations, 70 illustrations in colour
- Topics
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