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Methods in Molecular Biology

Bacterial Protein Secretion Systems

Methods and Protocols

Editors: Journet, Laure, Cascales, Eric (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 142,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7033-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 187,19 €
price for Spain (gross)
  • ISBN 978-1-4939-7031-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

Table of contents (38 chapters)

  • Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder

    Abby, Sophie S. (et al.)

    Pages 1-21

    Preview Buy Chapter 48,34 €
  • Protein Sorting Prediction

    Nielsen, Henrik

    Pages 23-57

    Preview Buy Chapter 48,34 €
  • Cell Fractionation

    Petiti, Melissa (et al.)

    Pages 59-64

    Preview Buy Chapter 48,34 €
  • Defining Lipoprotein Localisation by Fluorescence Microscopy

    Casabona, Maria Guillermina (et al.)

    Pages 65-74

    Preview Buy Chapter 48,34 €
  • Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

    Buddelmeijer, Nienke

    Pages 75-80

    Preview Buy Chapter 48,34 €

Buy this book

eBook 142,79 €
price for Spain (gross)
  • ISBN 978-1-4939-7033-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 187,19 €
price for Spain (gross)
  • ISBN 978-1-4939-7031-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Protein Secretion Systems
Book Subtitle
Methods and Protocols
Editors
  • Laure Journet
  • Eric Cascales
Series Title
Methods in Molecular Biology
Series Volume
1615
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7033-9
DOI
10.1007/978-1-4939-7033-9
Hardcover ISBN
978-1-4939-7031-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 528
Number of Illustrations
26 b/w illustrations, 70 illustrations in colour
Topics