Methods in Molecular Biology

Hemorrhagic Fever Viruses

Methods and Protocols

Editors: Salvato, Maria S. (Ed.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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About this book

This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Global Spread of Hemorrhagic Fever Viruses: Predicting Pandemics

    Gonzalez, Jean-Paul, M.D., Ph.D. (et al.)

    Pages 3-31

  • An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses

    Díaz, Francisco J. (et al.)

    Pages 33-41

  • Preliminary Classification of Novel Hemorrhagic Fever-Causing Viruses Using Sequence-Based PAirwise Sequence Comparison (PASC) Analysis

    Bào, Yīmíng (et al.)

    Pages 43-53

  • Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

    Montoya-Ruiz, Carolina (et al.)

    Pages 55-78

  • Diagnostics for Lassa Fever: Detecting Host Antibody Responses

    Salvato, Maria S. (et al.)

    Pages 79-88

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-1-4939-6981-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 155,99 €
price for Spain (gross)
  • ISBN 978-1-4939-6980-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 155,99 €
price for Spain (gross)
  • ISBN 978-1-4939-8358-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Hemorrhagic Fever Viruses
Book Subtitle
Methods and Protocols
Editors
  • Maria S. Salvato
Series Title
Methods in Molecular Biology
Series Volume
1604
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-6981-4
DOI
10.1007/978-1-4939-6981-4
Hardcover ISBN
978-1-4939-6980-7
Softcover ISBN
978-1-4939-8358-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 425
Number of Illustrations
9 b/w illustrations, 52 illustrations in colour
Topics