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ATM Kinase

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Sergei V. Kozlov

    1. University of Queensland Centre for Clinical Research (UQCCR), University of Queensland, Herston, Australia

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  • Includes cutting-edge methods and protocols for the study of ataxia-telangiectasia research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1599)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Assaying Radiosensitivity of Ataxia-Telangiectasia

    • Hailiang Hu, Shareef Nahas, Richard A. Gatti
    Pages 1-11

  3. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Technique

    • Amanda W. Kijas, Martin F. Lavin
    Pages 13-23

  4. ATM Gene Mutation Detection Techniques and Functional Analysis

    • Guillaume Rieunier, Catherine Dubois D’Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, Marc-Henri Stern
    Pages 25-42

  5. An HTRF® Assay for the Protein Kinase ATM

    • Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, Andrew Plater
    Pages 43-56

  6. ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using Cellomics™ ArrayScan VTI Platform

    • Catherine Bardelle, Joanna Boros
    Pages 57-70

  7. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci

    • Yi Chieh Lim
    Pages 71-84

  8. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy

    • Keiji Suzuki
    Pages 85-96

  9. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging

    • Shyam Nyati, Grant Young, Brian Dale Ross, Alnawaz Rehemtulla
    Pages 97-111

  10. Zn(II)–Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein

    • Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike
    Pages 113-126

  11. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry

    • Mark E. Graham, Martin F. Lavin, Sergei V. Kozlov
    Pages 127-144

  12. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)

    • Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, Ryo Otomo
    Pages 145-156

  13. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies

    • Yingli Sun, Fengxia Du
    Pages 157-162

  14. Identification of ATM-Interacting Proteins by Co-immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays

    • Amanda L. Bain, Janelle L. Harris, Kum Kum Khanna
    Pages 163-181

  15. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning Cytometry

    • Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, Zbigniew Darzynkiewicz
    Pages 183-196

  16. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling

    • Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, Amanda G. Paulovich
    Pages 197-213

  17. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation

    • Marina E. Borisova, Sebastian A. Wagner, Petra Beli
    Pages 215-227

  18. Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics

    • Ashley J. Waardenberg
    Pages 229-244

  19. ChIP Technique to Study Protein Dynamics at Defined DNA Double Strand Breaks

    • Jie Wen, Patrick Concannon
    Pages 245-262

  20. Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System

    • Rossana Piccinno, Marta Cipinska, Vassilis Roukos
    Pages 263-275
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Keywords

About this book

Ataxia–telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. 

Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.

Editors and Affiliations

  • University of Queensland Centre for Clinical Research (UQCCR), University of Queensland, Herston, Australia

    Sergei V. Kozlov

Bibliographic Information

  • Book Title: ATM Kinase

  • Book Subtitle: Methods and Protocols

  • Editors: Sergei V. Kozlov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6955-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6953-1Published: 06 May 2017

  • Softcover ISBN: 978-1-4939-8350-6Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6955-5Published: 05 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 437

  • Number of Illustrations: 24 b/w illustrations, 69 illustrations in colour

  • Topics: Human Genetics

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