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Peroxisomes

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Michael Schrader

    1. College of Life and Environmental Sciences, Biosciences, University of Exeter, Exeter, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1595)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation

    • Andreas Manner, Markus Islinger
    Pages 1-11

  3. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes

    • Miriam J. Schönenberger, Werner J. Kovacs
    Pages 13-26

  4. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays

    • Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, Jorge E. Azevedo
    Pages 27-35

  5. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces cerevisiae

    • Tobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, Ralf Erdmann
    Pages 37-44

  6. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and De Novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labeled Docosanoic Acid

    • Malu-Clair van de Beek, Inge M. E. Dijkstra, Stephan Kemp
    Pages 45-54

  7. Analysis of Plasmalogen Synthesis in Cultured Cells

    • Masanori Honsho, Yukio Fujiki
    Pages 55-61

  8. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

    • Janet Koster, Hans R. Waterham
    Pages 63-67

  9. siRNA-mediated Silencing of Peroxisomal Genes in Mammalian Cells

    • Tina A. Schrader, Michael Schrader
    Pages 69-79

  10. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals

    • Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, Sven Thoms
    Pages 81-92

  11. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3′-diaminobenzidine

    • H. Dariush Fahimi
    Pages 93-100

  12. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins

    • Karina Mildner, Dagmar Zeuschner
    Pages 101-111

  13. Detection and Immunolabeling of Peroxisomal Proteins

    • Tina A. Schrader, Markus Islinger, Michael Schrader
    Pages 113-130

  14. Labeling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago maydis

    • Sofia C. Guimarães, Sreedhar Kilaru, Michael Schrader, Martin Schuster
    Pages 131-150

  15. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes

    • Celien Lismont, Paul A. Walton, Marc Fransen
    Pages 151-164

  16. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress

    • Marc Fransen, Chantal Brees
    Pages 165-179

  17. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed

    • Luis F. Godinho, Michael Schrader
    Pages 181-189

  18. In Cellulo Approaches to Study Peroxisomal Protein Import – Yeast Immunofluorescence Microscopy

    • Tobias Hansen, Wolfgang Girzalsky, Ralf Erdmann
    Pages 191-196

  19. Blue Native PAGE: Applications to Study Peroxisome Biogenesis

    • Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki
    Pages 197-205

  20. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes

    • Yuqiong Liu, Masanori Honsho, Yukio Fujiki
    Pages 207-212
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Keywords

About this book

This volume provides easily accessible and comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Chapters detail valuable instructions, guidelines and protocols for molecular cell biologists, biochemists and biomedical researchers with an interest in peroxisome biology. Chapters in Peroxisomes: Methods and Protocols illustrate the isolation of peroxisomes, investigation of properties of membrane proteins, protocols to investigate and manipulate peroxisomes in cellular systems, detection of peroxisomes, including immunofluorescence, cytochemistry, cryo-immuno electron microscopy, and live cell imaging approaches.

Authoritative and practical, Peroxisomes: Methods and Protocols aims to be useful for those already working on peroxisomes as well as for those who would like to start working on this fascinating organelle.

Editors and Affiliations

  • College of Life and Environmental Sciences, Biosciences, University of Exeter, Exeter, United Kingdom

    Michael Schrader

Bibliographic Information

  • Book Title: Peroxisomes

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Schrader

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6937-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6935-7Published: 14 April 2017

  • Softcover ISBN: 978-1-4939-8344-5Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6937-1Published: 13 April 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 347

  • Number of Illustrations: 27 b/w illustrations, 36 illustrations in colour

  • Topics: Cell Biology

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