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Cholesterol Homeostasis

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Ingrid C. Gelissen

    1. Faculty of Pharmacy, The University of Sydney, Sydney, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Andrew J. Brown

    1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1583)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Overview of Cholesterol Homeostasis

    • Ingrid C. Gelissen, Andrew J. Brown
    Pages 1-6

  3. Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins

    • Coralie Di Scala, Jacques Fantini
    Pages 7-19

  4. Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review

    • Md. Jafurulla, Amitabha Chattopadhyay
    Pages 21-39

  5. Manipulating Cholesterol Status Within Cells

    • Winnie Luu, Ingrid C. Gelissen, Andrew J. Brown
    Pages 41-52

  6. Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells

    • Anke Loregger, Jessica K. Nelson, Noam Zelcer
    Pages 53-63

  7. The Use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolemia and Atherosclerosis

    • Eser J. Zerenturk, Anna C. Calkin
    Pages 65-72

  8. CRISPR/Cas9-Mediated Generation of Niemann–Pick C1 Knockout Cell Line

    • Ximing Du, Ivan Lukmantara, Hongyuan Yang
    Pages 73-83

  9. Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*

    • Jian Li, Peter L. Lee, Suzanne R. Pfeffer
    Pages 85-95

  10. Transport Assays for Sterol-Binding Proteins: Stopped-Flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein

    • Leslie A. McCauliff, Judith Storch
    Pages 97-110

  11. Synthesis and Live-Cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport

    • Maciej Modzel, Frederik W. Lund, Daniel Wüstner
    Pages 111-140

  12. Measurement of Cholesterol Transfer from Lysosome to Peroxisome Using an In Vitro Reconstitution Assay

    • Jie Luo, Ya-Cheng Liao, Jian Xiao, Bao-Liang Song
    Pages 141-161

  13. Measurement of Mitochondrial Cholesterol Import Using a Mitochondria-Targeted CYP11A1 Fusion Construct

    • Barry E. Kennedy, Mark Charman, Barbara Karten
    Pages 163-184

  14. Identifying Sterol Response Elements Within Promoters of Genes

    • Laura J. Sharpe, Andrew J. Brown
    Pages 185-191

  15. Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion

    • Lindsey L. Morris, Russell A. DeBose-Boyd
    Pages 193-199

  16. Determining the Topology of Membrane-Bound Proteins Using PEGylation

    • Vicky Howe, Andrew J. Brown
    Pages 201-210

  17. Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry

    • Anika V. Prabhu, Winnie Luu, Andrew J. Brown
    Pages 211-219

  18. Sterol Analysis by Quantitative Mass Spectrometry

    • Andrew M. Jenner, Simon H. J. Brown
    Pages 221-239

  19. Measurement of Rates of Cholesterol and Fatty Acid Synthesis In Vivo Using Tritiated Water

    • Adam M. Lopez, Jen-Chieh Chuang, Stephen D. Turley
    Pages 241-256

  20. Methods for Monitoring ABCA1-Dependent Sterol Release

    • Yoshio Yamauchi, Shinji Yokoyama, Ta-Yuan Chang
    Pages 257-273
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Keywords

About this book

This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell.  Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cholesterol Homeostasis: Methods and Protocols aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

Reviews

“The multicountry authors of this volume review present knowledge on the multiple basic roles of cholesterol in the living body. Each chapter details the methods of studies performed, backed by many references. For biologists, physiologists, biochemists.” (Zvi Laron, Pediatric Endocrinology Reviews (PER), Vol. 15 (1), September, 2017)

Editors and Affiliations

  • Faculty of Pharmacy, The University of Sydney, Sydney, Australia

    Ingrid C. Gelissen

  • School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia

    Andrew J. Brown

Bibliographic Information

  • Book Title: Cholesterol Homeostasis

  • Book Subtitle: Methods and Protocols

  • Editors: Ingrid C. Gelissen, Andrew J. Brown

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6875-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6873-2Published: 17 February 2017

  • Softcover ISBN: 978-1-4939-8325-4Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6875-6Published: 15 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 300

  • Number of Illustrations: 27 b/w illustrations, 37 illustrations in colour

  • Topics: Cell Biology

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