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Auxins and Cytokinins in Plant Biology

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Thomas Dandekar

    1. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany

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  2. Muhammad Naseem

    1. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Wurzburg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1569)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Methodological Advances in Auxin and Cytokinin Biology

    • Andrej Hurný, Eva Benková
    Pages 1-29

  3. Analytical Determination of Auxins and Cytokinins

    • Petre I. Dobrev, Klára Hoyerová, Jan Petrášek
    Pages 31-39

  4. Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae–Arabidopsis thaliana Interaction

    • Jutta Ludwig-Müller, Susann Auer, Sabine Jülke, Sabine Marschollek
    Pages 41-60

  5. Microbial Manipulation of Auxins and Cytokinins in Plants

    • Anwar Hussain, Ihsan Ullah, Shahida Hasnain
    Pages 61-72

  6. A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling

    • Rocío Olmo, Ana Cláudia Silva, Fernando E. Díaz-Manzano, Javier Cabrera, Carmen Fenoll, Carolina Escobar
    Pages 73-81

  7. Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity

    • Martin Kaltdorf, Thomas Dandekar, Muhammad Naseem
    Pages 83-92

  8. Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK)

    • Pallavi Singh, Alok Krishna Sinha
    Pages 93-100

  9. Quantification of Cytokinin Levels and Responses in Abiotic Stresses

    • Alfonso Albacete
    Pages 101-111

  10. Assessment of Cytokinin-Induced Immunity Through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana

    • Ruth A. Watson, Cristiana T. Argueso
    Pages 113-126

  11. Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies

    • Martin Schäfer, Stefan Meldau
    Pages 127-139

  12. Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface

    • Muhammad Naseem, Shabana Shams, Thomas Roitsch
    Pages 141-150

  13. Analyzing Cytokinin Responses During Plant-Nematode Interactions

    • Florian M. W. Grundler, Shahid Siddique
    Pages 151-158

  14. Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin

    • Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, Nazeer Ahmed
    Pages 159-163

  15. A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks

    • Meik Kunz, Thomas Dandekar, Muhammad Naseem
    Pages 165-173

  16. Monitoring of Crosstalk Between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots

    • Víctor Carrasco Loba, Marta-Marina Pérez Alonso, Stephan Pollmann
    Pages 175-185

  17. High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation

    • Nora Wehner, Jörn Herfert, Wolfgang Dröge-Laser, Christoph Weiste
    Pages 187-202

  18. Back Matter

    Pages 203-204
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Keywords

About this book

This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.

 

Editors and Affiliations

  • Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    Thomas Dandekar

  • Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Wurzburg, Germany

    Muhammad Naseem

Bibliographic Information

  • Book Title: Auxins and Cytokinins in Plant Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Dandekar, Muhammad Naseem

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6831-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6829-9Published: 10 March 2017

  • Softcover ISBN: 978-1-4939-8311-7Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6831-2Published: 06 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 204

  • Number of Illustrations: 8 b/w illustrations, 23 illustrations in colour

  • Topics: Plant Sciences

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