Methods in Molecular Biology

Signal Transduction Immunohistochemistry

Methods and Protocols

Editors: Kalyuzhny, Alexander E. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods on digital imaging techniques, preservation of tissue targets, multicolor detection, flow cytometry, lipophagy analysis, apoptosis, and the combination of IHC with in situ hybridization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Signal Transduction Immunohistochemistry: Methods and Protocols, Second Edition is a valuable resource to both novices and experts in other fields of biomedical research who need advice on IHC protocols to study signal transduction. This book will also be useful for researchers in academia, government labs, and the biotech industry.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications

    Brumbaugh, Kathy (et al.)

    Pages 1-40

  • Antibodies That Work Again and Again and Again

    Simpson, Kameron (et al.)

    Pages 41-59

  • Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods

    Kurien, Biji T. (et al.)

    Pages 61-73

  • Acquisition and Post-Processing of Immunohistochemical Images

    Sedgewick, Jerry

    Pages 75-106

  • Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction

    Kalra, Jessica (et al.)

    Pages 107-125

Buy this book

eBook 118,99 €
price for Spain (gross)
  • ISBN 978-1-4939-6759-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-4939-6757-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 145,59 €
price for Spain (gross)
  • ISBN 978-1-4939-8294-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Signal Transduction Immunohistochemistry
Book Subtitle
Methods and Protocols
Editors
  • Alexander E. Kalyuzhny
Series Title
Methods in Molecular Biology
Series Volume
1554
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6759-9
DOI
10.1007/978-1-4939-6759-9
Hardcover ISBN
978-1-4939-6757-5
Softcover ISBN
978-1-4939-8294-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 290
Number of Illustrations
22 b/w illustrations, 45 illustrations in colour
Topics