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Antimicrobial Peptides

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Paul R. Hansen

    1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1548)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Properties, Design, Synthesis and Characterization of AMPs

    1. Front Matter

      Pages 1-1
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    2. Antimicrobial Peptides: An Introduction

      • Evan F. Haney, Sarah C. Mansour, Robert E. W. Hancock
      Pages 3-22

    3. Tools for Designing Amphipathic Helical Antimicrobial Peptides

      • Davor Juretić, Damir Vukičević, Alessandro Tossi
      Pages 23-34

    4. Chemical Synthesis of Antimicrobial Peptides

      • Lena Münzker, Alberto Oddo, Paul R. Hansen
      Pages 35-49

    5. Microwave-Assisted Synthesis of Antimicrobial Peptides

      • Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, Thavendran Govender
      Pages 51-59

    6. High-Performance Liquid Chromatography and Mass Spectrometry-Based Design of Proteolytically Stable Antimicrobial Peptides

      • Mojtaba Bagheri, Robert E. W. Hancock
      Pages 61-71

    7. Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR

      • Reinhard Wimmer, Lars Erik Uggerhøj
      Pages 73-88

    8. Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides

      • Jiongyu Liu, Jianping Jiang
      Pages 89-99

  3. Studying Interactions of AMPs with Model Membranes and Bacteria

    1. Front Matter

      Pages 101-101
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    2. Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptide–Lipid Bilayer Interactions

      • Shima Arasteh, Mojtaba Bagheri
      Pages 103-118

    3. Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides

      • Mauricio Arias, Elmar J. Prenner, Hans J. Vogel
      Pages 119-140

    4. Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides

      • Mauricio Arias, Hans J. Vogel
      Pages 141-157

    5. Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption

      • Kasper Kristensen, Jonas R. Henriksen, Thomas L. Andresen
      Pages 159-180

    6. Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action

      • Bimal Jana, Kristin Renee Baker, Luca Guardabassi
      Pages 181-190

    7. Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides

      • Gabriel Del Rio, Edda Klipp, Andreas Herrmann
      Pages 191-199

    8. Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA

      • Peter Fojan, Leonid Gurevich
      Pages 201-215

    9. Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR

      • Nury P. Santisteban, Michael R. Morrow, Valerie Booth
      Pages 217-230

    10. Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR

      • Rickey Hicks
      Pages 231-245

    11. Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism

      • Concetta Avitabile, Luca Domenico D’Andrea, Alessandra Romanelli
      Pages 247-253
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Keywords

About this book

This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological activities of AMPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Antimicrobial Peptides: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Editors and Affiliations

  • Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Paul R. Hansen

Bibliographic Information

  • Book Title: Antimicrobial Peptides

  • Book Subtitle: Methods and Protocols

  • Editors: Paul R. Hansen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6737-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6735-3Published: 24 December 2016

  • Softcover ISBN: 978-1-4939-8287-5Published: 12 July 2018

  • eBook ISBN: 978-1-4939-6737-7Published: 22 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 439

  • Number of Illustrations: 38 b/w illustrations, 49 illustrations in colour

  • Topics: Microbiology

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