Methods in Molecular Biology

Metagenomics

Methods and Protocols

Editors: Streit, Wolfgang, Daniel, Rolf (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.

 

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Construction of Small-Insert and Large-Insert Metagenomic Libraries

    Pages 1-12

    Simon, Carola (et al.)

  • Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies

    Pages 13-22

    Schneider, Dominik (et al.)

  • Construction and Screening of Marine Metagenomic Large Insert Libraries

    Pages 23-42

    Weiland-Bräuer, Nancy (et al.)

  • Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

    Pages 43-55

    Glaring, Mikkel A. (et al.)

  • DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms

    Pages 57-74

    Jameson, Eleanor (et al.)

Buy this book

eBook 85,59 €
price for Spain (gross)
  • ISBN 978-1-4939-6691-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 103,99 €
price for Spain (gross)
  • ISBN 978-1-4939-6689-9
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 103,99 €
price for Spain (gross)
  • ISBN 978-1-4939-8274-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Metagenomics
Book Subtitle
Methods and Protocols
Editors
  • Wolfgang Streit
  • Rolf Daniel
Series Title
Methods in Molecular Biology
Series Volume
1539
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6691-2
DOI
10.1007/978-1-4939-6691-2
Hardcover ISBN
978-1-4939-6689-9
Softcover ISBN
978-1-4939-8274-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 303
Number of Illustrations
24 b/w illustrations, 24 illustrations in colour
Topics