High-Throughput Glycomics and Glycoproteomics
Methods and Protocols
Editors: Lauc, Gordan, Wuhrer, Manfred (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. The book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughout with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, High-Throughout Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.
- Table of contents (19 chapters)
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Ubiquitous Importance of Protein Glycosylation
Pages 1-12
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Laboratory Experimental Design for a Glycomic Study
Pages 13-19
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High-Throughput Analysis of the IgG N-Glycome by UPLC-FLR
Pages 21-29
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High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS
Pages 31-47
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Sialic Acid Derivatization for the Rapid Subclass- and Sialic Acid Linkage-Specific MALDI-TOF-MS Analysis of IgG Fc-Glycopeptides
Pages 49-62
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Table of contents (19 chapters)
- Download Preface 1 PDF (71.5 KB)
- Download Sample pages 2 PDF (150.9 KB)
- Download Table of contents PDF (79 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- High-Throughput Glycomics and Glycoproteomics
- Book Subtitle
- Methods and Protocols
- Editors
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- Gordan Lauc
- Manfred Wuhrer
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1503
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-6493-2
- DOI
- 10.1007/978-1-4939-6493-2
- Hardcover ISBN
- 978-1-4939-6491-8
- Softcover ISBN
- 978-1-4939-8217-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 274
- Number of Illustrations
- 9 b/w illustrations, 42 illustrations in colour
- Topics
