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ERK Signaling

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Gerardo Jimenez

    1. Institut de Biologia Molecular de Barcelona-CSIC, Parc Científic de Barcelona, Barcelona, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1487)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. How Genetics Has Helped Piece Together the MAPK Signaling Pathway

    • Dariel Ashton-Beaucage, Marc Therrien
    Pages 1-21

  3. In Vitro Enzyme Kinetics Analysis of EGFR

    • Zhihong Wang, Christine Candelora
    Pages 23-33

  4. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells

    • Nobuo Yoshimoto, Shun’ichi Kuroda
    Pages 35-52

  5. Structural Studies of ERK2 Protein Complexes

    • Johannes F. Weijman, Stefan J. Riedl, Peter D. Mace
    Pages 53-63

  6. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk

    • Tal Goshen-Lago, Dganit Melamed, Arie Admon, David Engelberg
    Pages 65-88

  7. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues

    • Carme Caelles, Carles Bayod, Melisa Morcillo
    Pages 89-98

  8. Detection and Functional Analysis of SUMO-Modified MEK

    • Yuji Kubota, Mutsuhiro Takekawa
    Pages 99-111

  9. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag

    • Liu Yang, Alexey Veraksa
    Pages 113-126

  10. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates

    • Rona Grossman, Ze’ev Paroush
    Pages 127-135

  11. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE

    • Hidetaka Kosako, Kou Motani
    Pages 137-149

  12. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation

    • Lorena Agudo-Ibañez, Piero Crespo, Berta Casar
    Pages 151-162

  13. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions

    • Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo
    Pages 163-174

  14. The Nuclear Translocation of ERK

    • Denise A. Berti, Rony Seger
    Pages 175-194

  15. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)

    • Zijian Tang, Chengkai Dai
    Pages 195-201

  16. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors

    • Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, Olivier Pertz
    Pages 203-221

  17. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers

    • Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, Masahiro Sokabe
    Pages 223-234

  18. Co-culture Activation of MAP Kinase in Drosophila S2 Cells

    • Josefa Steinhauer
    Pages 235-241

  19. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling

    • Marc K. Saba-El-Leil, Christophe Frémin, Sylvain Meloche
    Pages 243-253

  20. 3D Organotypic Culture Model to Study Components of ERK Signaling

    • Athina-Myrto Chioni, Rabia Tayba Bajwa, Richard Grose
    Pages 255-267
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Keywords

About this book

This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling.

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Editors and Affiliations

  • Institut de Biologia Molecular de Barcelona-CSIC, Parc Científic de Barcelona, Barcelona, Spain

    Gerardo Jimenez

Bibliographic Information

  • Book Title: ERK Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Gerardo Jimenez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6424-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6422-2Published: 06 December 2016

  • Softcover ISBN: 978-1-4939-8195-3Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6424-6Published: 06 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 440

  • Number of Illustrations: 19 b/w illustrations, 21 illustrations in colour

  • Topics: Biochemistry, general

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