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Methods in Molecular Biology

Cilia

Methods and Protocols

Editors: Satir, Peter, Christensen, Søren Tvorup (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 80,24 €
price for Spain (gross)
  • ISBN 978-1-4939-3789-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover 98,79 €
price for Spain (gross)
Softcover 98,79 €
price for Spain (gross)
About this book

This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.

 

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Methods for Studying Ciliary Import Mechanisms

    Pages 1-14

    Takao, Daisuke (et al.)

  • Targeting of ASH Domain-Containing Proteins to the Centrosome

    Pages 15-33

    Verdier, Patricia (et al.)

  • Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy

    Pages 35-51

    Ghossoub, Rania (et al.)

  • Methods to Study Interactions Between Ciliogenesis and Autophagy

    Pages 53-67

    Satir, Birgit Hegner, Ph.D. (et al.)

  • Recombinant Reconstitution and Purification of the IFT-B Core Complex from Chlamydomonas reinhardtii

    Pages 69-82

    Taschner, Michael (et al.)

Buy this book

eBook 80,24 €
price for Spain (gross)
  • ISBN 978-1-4939-3789-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover 98,79 €
price for Spain (gross)
Softcover 98,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Cilia
Book Subtitle
Methods and Protocols
Editors
  • Peter Satir
  • Søren Tvorup Christensen
Series Title
Methods in Molecular Biology
Series Volume
1454
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3789-9
DOI
10.1007/978-1-4939-3789-9
Hardcover ISBN
978-1-4939-3787-5
Softcover ISBN
978-1-4939-8139-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 259
Number of Illustrations
15 b/w illustrations, 39 illustrations in colour
Topics