Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1451)
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Table of contents (26 protocols)
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Front Matter
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Genetics and Genomics
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Front Matter
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Disease Models and Mechanism
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Front Matter
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Neuroscience
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Front Matter
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About this book
This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish, applications of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection and host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuits and their role in generating behavior. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful complement to the first book for new and experienced zebrafish researcher alike.
Editors and Affiliations
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Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan
Koichi Kawakami
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Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom
E. Elizabeth Patton
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Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal
Michael Orger
Bibliographic Information
Book Title: Zebrafish
Book Subtitle: Methods and Protocols
Editors: Koichi Kawakami, E. Elizabeth Patton, Michael Orger
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3771-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3769-1Published: 27 July 2016
Softcover ISBN: 978-1-4939-8134-2Published: 31 May 2018
eBook ISBN: 978-1-4939-3771-4Published: 27 July 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 373
Number of Illustrations: 15 b/w illustrations, 64 illustrations in colour
Topics: Biochemistry, general