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Methods in Molecular Biology

Gap Junction Protocols

Editors: Vinken, Mathieu, Johnstone, Scott R. (Eds.)

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  • Includes cutting-edge methods and protocols involving gap junctions
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 80,24 €
price for Spain (gross)
  • ISBN 978-1-4939-3664-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover 135,19 €
price for Spain (gross)
Softcover 98,79 €
price for Spain (gross)
About this book

Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

    Pages 1-19

    Maes, Michaël (et al.)

  • DNA Methylation Analysis of Human Tissue-Specific Connexin Genes

    Pages 21-36

    Liu, Xiaoming (et al.)

  • Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis

    Pages 37-53

    Willebrords, Joost (et al.)

  • Immunohisto- and Cytochemistry Analysis of Connexins

    Pages 55-70

    Cogliati, Bruno (et al.)

  • Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells

    Pages 71-82

    Good, Miranda E. (et al.)

Buy this book

eBook 80,24 €
price for Spain (gross)
  • ISBN 978-1-4939-3664-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover 135,19 €
price for Spain (gross)
Softcover 98,79 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Gap Junction Protocols
Editors
  • Mathieu Vinken
  • Scott R. Johnstone
Series Title
Methods in Molecular Biology
Series Volume
1437
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-3664-9
DOI
10.1007/978-1-4939-3664-9
Hardcover ISBN
978-1-4939-3662-5
Softcover ISBN
978-1-4939-8110-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 235
Number of Illustrations
11 b/w illustrations, 34 illustrations in colour
Topics