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  • © 2016

Auditory and Vestibular Research

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1427)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
  2. RNA Delivery and Extraction Protocols

    1. Front Matter

      Pages 1-1
    2. A Rapid, Cost-Effective Method to Prepare Recombinant Adeno-Associated Virus for Efficient Gene Transfer to the Developing Mouse Inner Ear

      • Michelle M. Gomes, Lingyan Wang, Han Jiang, Christoph A. Kahl, John V. Brigande
      Pages 43-57
    3. RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays

      • Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, Daniel Ramirez-Gordillo, Elba E. Serrano
      Pages 73-92
  3. Protein Protocols

    1. Front Matter

      Pages 93-93
    2. Protein Quantitation of the Developing Cochlea Using Mass Spectrometry

      • Lancia N. F. Darville, Bernd H. A. Sokolowski
      Pages 135-148
    3. Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins

      • Dennis G. Drescher, Selvakumar Dakshnamurthy, Marian J. Drescher, Neeliyath A. Ramakrishnan
      Pages 165-187
    4. The Single-Molecule Approach to Membrane Protein Stoichiometry

      • Michael G. Nichols, Richard Hallworth
      Pages 189-199
  4. Imaging Protocols

    1. Front Matter

      Pages 201-201
    2. Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue

      • Federico Ceriani, Catalin D. Ciubotaru, Mario Bortolozzi, Fabio Mammano
      Pages 223-241
    3. Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments

      • Bernd Fritzsch, Jeremy S. Duncan, Jennifer Kersigo, Brian Gray, Karen L. Elliott
      Pages 243-262
    4. Rapid and Semi-automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks

      • Paul S. Holcomb, Michael Morehead, Gianfranco Doretto, Peter Chen, Stuart Berg, Stephen Plaza et al.
      Pages 277-290

About this book

This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughput screening, and physiology.  Chapters on physiology cover techniques that include optical coherence tomography, patch clamping, and photostimulation of caged neurotransmitters.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Auditory and Vestibular Research: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • College of Medicine, University of South Florida, Tampa, USA

    Bernd Sokolowski

Bibliographic Information

  • Book Title: Auditory and Vestibular Research

  • Book Subtitle: Methods and Protocols

  • Editors: Bernd Sokolowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3615-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3613-7Published: 04 June 2016

  • Softcover ISBN: 978-1-4939-8095-6Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3615-1Published: 03 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 547

  • Number of Illustrations: 31 b/w illustrations, 89 illustrations in colour

  • Topics: Molecular Medicine, Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access