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Vaccine Design

Methods and Protocols: Volume 1: Vaccines for Human Diseases

  • Book
  • © 2016

Overview

Editors:
  1. Sunil Thomas

    1. Lankenau Institute for Medical Research, Wynnewood, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1403)

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Table of contents (50 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Vaccines: Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Clinical Impact of Vaccine Development

      • Puja H. Nambiar, Alejandro Delgado Daza, Lawrence L. Livornese Jr
      Pages 3-39

    3. Future Challenges for Vaccinologists

      • Sunil Thomas, Rima Dilbarova, Rino Rappuoli
      Pages 41-55

    4. Principles of Vaccination

      • Fred Zepp
      Pages 57-84

  3. Trends in Vaccinology

    1. Front Matter

      Pages 85-85
      Download chapter PDF

    2. Reverse Vaccinology: The Pathway from Genomes and Epitope Predictions to Tailored Recombinant Vaccines

      • Marcin Michalik, Bardya Djahanshiri, Jack C. Leo, Dirk Linke
      Pages 87-106

    3. Systems Vaccinology: Applications, Trends, and Perspectives

      • Johannes Sollner
      Pages 107-130

    4. Proteomic Monitoring of B Cell Immunity

      • Radwa Ewaisha, Karen S. Anderson
      Pages 131-152

  4. Vaccines for Human Viral Diseases

    1. Front Matter

      Pages 153-153
      Download chapter PDF

    2. Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization, and Protection Studies

      • Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje, Claudio Prieto
      Pages 155-166

    3. Analytic Vaccinology: Antibody-Driven Design of a Human Cytomegalovirus Subunit Vaccine

      • Anna Kabanova, Daniele Lilleri
      Pages 167-186

    4. Generation of a Single-Cycle Replicable Rift Valley Fever Vaccine

      • Shin Murakami, Kaori Terasaki, Shinji Makino
      Pages 187-206

    5. Application of Droplet Digital PCR to Validate Rift Valley Fever Vaccines

      • Hoai J. Ly, Nandadeva Lokugamage, Tetsuro Ikegami
      Pages 207-220

    6. Methods to Evaluate Novel Hepatitis C Virus Vaccines

      • Gustaf Ahlén, Lars Frelin
      Pages 221-244

    7. Designing Efficacious Vesicular Stomatitis Virus-Vectored Vaccines Against Ebola Virus

      • Gary Wong, Xiangguo Qiu
      Pages 245-257

    8. Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice

      • Maria Malm, Kirsi Tamminen, Vesna Blazevic
      Pages 259-268

    9. Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein Plus Delta Inulin Adjuvant

      • Clifton McPherson, Richard Chubet, Kathy Holtz, Yoshikazu Honda-Okubo, Dale Barnard, Manon Cox et al.
      Pages 269-284

    10. Generation and Characterization of a Chimeric Tick-Borne Encephalitis Virus Attenuated Strain ChinTBEV

      • Hong-Jiang Wang, Xiao-Feng Li, Cheng-Feng Qin
      Pages 285-293

    11. Single-Vector, Single-Injection Recombinant Vesicular Stomatitis Virus Vaccines Against High-Containment Viruses

      • Michael A. Whitt, Thomas W. Geisbert, Chad E. Mire
      Pages 295-311
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Keywords

About this book

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 1: Vaccines for Human Diseases has an introductory section on how vaccines impacted diseases, the immunological mechanism of vaccines, future challenges for vaccinologists, and current trends in vaccinology. The design of human vaccines for viral, bacterial, fungal, parasitic and prion diseases as well as vaccines for drug abuse, allergy, and tumor vaccines are also described in this volume. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 1: Vaccines

for Human Diseases aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Lankenau Institute for Medical Research, Wynnewood, USA

    Sunil Thomas

Bibliographic Information

  • Book Title: Vaccine Design

  • Book Subtitle: Methods and Protocols: Volume 1: Vaccines for Human Diseases

  • Editors: Sunil Thomas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3387-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3385-3Published: 14 April 2016

  • Softcover ISBN: 978-1-4939-8038-3Published: 19 April 2018

  • eBook ISBN: 978-1-4939-3387-7Published: 13 April 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 873

  • Number of Illustrations: 42 b/w illustrations, 89 illustrations in colour

  • Topics: Immunology, Vaccine

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