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PTEN

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Leonardo Salmena

    1. University of Toronto, Princess Margaret Cancer Centre, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Vuk Stambolic

    1. University of Toronto, Princess Margaret Cancer Centre, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1388)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introductory Chapters

    1. Front Matter

      Pages 1-1
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    2. PTEN: History of a Tumor Suppressor

      • Leonardo Salmena
      Pages 3-11

    3. PTEN at 18: Still Growing

      • Olena Gorbenko, Vuk Stambolic
      Pages 13-19

  3. Methods to Detect PTEN in Disease

    1. Front Matter

      Pages 21-21
      Download chapter PDF

    2. Immunopathologic Assessment of PTEN Expression

      • Mireia Castillo-Martin, Tin Htwe Thin, Ana Collazo Lorduy, Carlos Cordon-Cardo
      Pages 23-37

    3. Measurement of PTEN by Flow Cytometry

      • John F. Woolley, Leonardo Salmena
      Pages 39-51

    4. Detecting PTEN and PI3K Signaling in Brain

      • Guo Zhu, Suzanne J. Baker
      Pages 53-62

    5. Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome

      • Joanne Ngeow, Charis Eng
      Pages 63-73

    6. Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis

      • Cynthia T. Luk, Stephanie A. Schroer, Minna Woo
      Pages 75-91

  4. Methods to Study PTEN Regulation

    1. Front Matter

      Pages 93-93
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    2. Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET

      • Stanislas Misticone, Evelyne Lima-Fernandes, Mark G. H. Scott
      Pages 95-110

    3. Methods for the Identification of PTEN-Targeting MicroRNAs

      • Andrea Tuccoli, Marianna Vitiello, Andrea Marranci, Francesco Russo, Laura Poliseno
      Pages 111-138

    4. Posttranscriptional Regulation of PTEN by Competing Endogenous RNAs

      • Yvonne Tay, Pier Paolo Pandolfi
      Pages 139-154

    5. In Cell and In Vitro Assays to Measure PTEN Ubiquitination

      • Amit Gupta, Helene Maccario, Nisha Kriplani, Nicholas R. Leslie
      Pages 155-165

  5. Methods to Study PTEN Localization

    1. Front Matter

      Pages 167-167
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    2. Assessing PTEN Subcellular Localization

      • Anabel Gil, José I. López, Rafael Pulido
      Pages 169-186

    3. Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum

      • Sonia Missiroli, Claudia Morganti, Carlotta Giorgi, Paolo Pinton
      Pages 187-212

  6. Methods to Study PTEN Structure

    1. Front Matter

      Pages 213-213
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    2. Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry

      • Glenn R. Masson, John E. Burke, Roger L. Williams
      Pages 215-230
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Keywords

About this book

This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.

Editors and Affiliations

  • University of Toronto, Princess Margaret Cancer Centre, Toronto, Canada

    Leonardo Salmena, Vuk Stambolic

About the editors

Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9

Bibliographic Information

  • Book Title: PTEN

  • Book Subtitle: Methods and Protocols

  • Editors: Leonardo Salmena, Vuk Stambolic

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3299-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3297-9Published: 11 December 2015

  • Softcover ISBN: 978-1-4939-8015-4Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3299-3Published: 11 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 324

  • Number of Illustrations: 28 b/w illustrations, 17 illustrations in colour

  • Topics: Cancer Research, Human Genetics

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