Receptor and Ion Channel Detection in the Brain
Methods and Protocols
Editors: Lujan, Rafael, Ciruela, Francisco (Eds.)
Free Preview- Includes cutting-edge methods and key protocols to study receptors and ion channels
- Provides step-by-step detail essential for reproducible results
- Contains well-organized themes, key notes and expert implementation advice
- Includes methods applicable to the basic research and clinical laboratories
- Suitable for both novice and experienced investigators
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- About this book
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Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.
- Table of contents (29 chapters)
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Production of High-Quality Antibodies for the Study of Receptors and Ion Channels
Pages 3-18
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Co-immunoprecipitation from Brain
Pages 19-29
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Subsynaptic Membrane Fractionation
Pages 31-37
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Investigation of Neurotransmitter Receptors in Brain Slices Using Cell Surface Biotinylation
Pages 39-48
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Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Pages 49-58
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Table of contents (29 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Receptor and Ion Channel Detection in the Brain
- Book Subtitle
- Methods and Protocols
- Editors
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- Rafael Lujan
- Francisco Ciruela
- Series Title
- Neuromethods
- Series Volume
- 110
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-3064-7
- DOI
- 10.1007/978-1-4939-3064-7
- Hardcover ISBN
- 978-1-4939-3063-0
- Softcover ISBN
- 978-1-4939-7983-7
- Series ISSN
- 0893-2336
- Edition Number
- 1
- Number of Pages
- XVIII, 475
- Topics
