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Phospho-Proteomics

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Louise von Stechow

    1. University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1355)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Techniques for Tagging Phospho-Moieties

    1. Front Matter

      Pages 1-1
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    2. Thiol-ene-Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins

      • Kaelyn E. Wilke, Erin E. Carlson
      Pages 3-15

    3. Phosphopeptide Detection with Biotin-Labeled Phos-tag

      • Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
      Pages 17-29

    4. Phosphopeptide Enrichment by Covalent Chromatography After Solid Phase Derivatization of Protein Digests on Reversed Phase Supports

      • Heinz Nika, Ruth Hogue Angeletti, David H. Hawke
      Pages 31-50

  3. Labeling Techniques for Quantitative Phosphoproteomics

    1. Front Matter

      Pages 51-51
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    2. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics

      • Lei Cheng, Trairak Pisitkun, Mark A. Knepper, Jason D. Hoffert
      Pages 53-70

    3. Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles

      • Daniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, Sebastian Lourido
      Pages 71-84

    4. Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)

      • Ryohei Narumi, Takeshi Tomonaga
      Pages 85-101

  4. Phosphopeptide Enrichment Strategies

    1. Front Matter

      Pages 103-103
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    2. Enrichment Strategies in Phosphoproteomics

      • Alexander Leitner
      Pages 105-121

    3. Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography

      • Tine E. Thingholm, Martin R. Larsen
      Pages 123-133

    4. The Use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides

      • Tine E. Thingholm, Martin R. Larsen
      Pages 135-146

    5. Sequential Elution from IMAC (SIMAC): An Efficient Method for Enrichment and Separation of Mono- and Multi-phosphorylated Peptides

      • Tine E. Thingholm, Martin R. Larsen
      Pages 147-160

    6. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation

      • Kasper Engholm-Keller, Martin R. Larsen
      Pages 161-177

    7. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage

      • Tanveer S. Batth, Jesper V. Olsen
      Pages 179-192

    8. Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview

      • Íris L. Batalha, Ana Cecília A. Roque
      Pages 193-209

  5. Phosphoprotein Analysis by Mass Spectrometry

    1. Front Matter

      Pages 211-211
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    2. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics

      • Chao Han, Pingfang Yang
      Pages 213-223

    3. Variable Digestion Strategies for Phosphoproteomics Analysis

      • Humberto Gonczarowska-Jorge, Margherita Dell’Aica, Clarissa Dickhut, René P. Zahedi
      Pages 225-239
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Keywords

About this book

This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark

    Louise von Stechow

Bibliographic Information

  • Book Title: Phospho-Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Louise von Stechow

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3049-4

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3048-7Published: 20 November 2015

  • Softcover ISBN: 978-1-4939-5001-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3049-4Published: 19 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 364

  • Topics: Protein Science, Proteomics

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