- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each protocol in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.
- Table of contents (18 chapters)
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Library Construction for Mutation Identification by Whole-Genome Sequencing
Pages 1-9
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Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes
Pages 11-21
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Genetic Methods for Cellular Manipulations in C. elegans
Pages 23-38
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A Fusion PCR Method for Expressing Genetic Tools in C. elegans
Pages 39-47
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Transposon-Assisted Genetic Engineering with Mos1-Mediated Single-Copy Insertion (MosSCI)
Pages 49-58
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Table of contents (18 chapters)
- Download Preface 1 PDF (73 KB)
- Download Sample pages 1 PDF (306.7 KB)
- Download Table of contents PDF (48.2 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- C. elegans
- Book Subtitle
- Methods and Applications
- Editors
-
- David Biron
- Gal Haspel
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1327
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2842-2
- DOI
- 10.1007/978-1-4939-2842-2
- Hardcover ISBN
- 978-1-4939-2841-5
- Softcover ISBN
- 978-1-4939-4892-5
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XII, 252
- Number of Illustrations
- 13 b/w illustrations, 44 illustrations in colour
- Topics
