Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1271)
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Table of contents (25 protocols)
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Front Matter
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Historical Overview
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Front Matter
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Rhodopsin Expression, Regeneration, and Purification for Structural Studies
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Front Matter
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Structure–Function Characterization of Rhodopsin
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Front Matter
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Rhodopsin Supramolecular Organization and Its Complexes with Partner Proteins
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Front Matter
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Keywords
About this book
This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification protocols of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers stabilizing rhodopsin function properties, and finally describing recently developed methods to study structural dynamics of rhodopsin activation and its mechanistic properties. Subsequently, chapters underline various techniques that have been developed to visualize the rhodopsin dimer and to study its functional significance. Â The next few chapters highlight cutting-edge imaging techniques of photoreceptors, rhodopsin trafficking, and its diffusion within signaling membranes. Finally the book concludes with recent developments that could be potentially beneficial in patient treatments, and treatment strategies for retinal degenerative diseases. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Timely and practical, Rhodopsin: Methods and Protocols reaches out to researchers and health practitioners, and provides timely protocol useful for studying structural and functional properties of rhodopsin.
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Rhodopsin
Book Subtitle: Methods and Protocols
Editors: Beata Jastrzebska
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2330-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2329-8Published: 20 February 2015
Softcover ISBN: 978-1-4939-4936-6Published: 05 October 2016
eBook ISBN: 978-1-4939-2330-4Published: 19 February 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 414
Number of Illustrations: 23 b/w illustrations, 71 illustrations in colour
Topics: Cell Biology, Proteomics, Membrane Biology