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In Situ Hybridization Methods

  • Book
  • © 2015

Overview

Editors:
  1. Giselbert Hauptmann

    1. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 99)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xx
    Download chapter PDF

  2. FFPE Sections

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Optimization of In Situ Hybridization and Co-expression Analysis in the Central Nervous System

      • Louisa Mezache, Madison Mikhail, Matteo Fassan, Marco Chilosi, Gerard J. Nuovo
      Pages 3-25

  3. Model Organisms

    1. Front Matter

      Pages 27-27
      Download chapter PDF

    2. In Situ Hybridization Methods for RNA Visualization in C. elegans

      • Gina Broitman-Maduro, Morris F. Maduro
      Pages 29-44

    3. Multicolored Visualization of Transcript Distributions in Drosophila Embryos

      • Iris Söll, Giselbert Hauptmann
      Pages 45-59

    4. Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos

      • José Luis Ferran, Abdelmalik Ayad, Paloma Merchán, Nicanor Morales-Delgado, Luisa Sánchez-Arrones, Antonia Alonso et al.
      Pages 61-82

    5. Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections

      • José Luis Ferran, Abdelmalik Ayad, Paloma Merchán, Nicanor Morales-Delgado, Luisa Sánchez-Arrones, Antonia Alonso et al.
      Pages 83-107

    6. Combined Radioactive and Nonradioactive Double In Situ Hybridization (DISH)

      • Jong-Hyun Son, Ursula H. Winzer-Serhan
      Pages 109-126

    7. One FISH, dFISH, Three FISH: Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians

      • David D. R. Brown, Bret J. Pearson
      Pages 127-150

    8. Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos

      • Edgar M. Pera, Helena Acosta, Nadège Gouignard, Maria Climent
      Pages 151-167

  5. Evo-Devo

    1. Front Matter

      Pages 247-247
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    2. Analysis of Embryonic Gene Expression Patterns in the Hagfish

      • Yasuhiro Oisi, Osamu Kakitani, Shigeru Kuratani, Kinya G. Ota
      Pages 249-262

    3. Gene Expression Analysis of Lamprey Embryos

      • Fumiaki Sugahara, Yasunori Murakami, Shigeru Kuratani
      Pages 263-278

    4. Detection of mRNA and microRNA Expression in Basal Chordates, Amphioxus and Ascidians

      • Simona Candiani, Greta Garbarino, Mario Pestarino
      Pages 279-292

    5. Analysis of Gene Expression in Neurons and Synapses by Multi-color In Situ Hybridization

      • Leonid L. Moroz, Andrea B. Kohn
      Pages 293-317
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Keywords

About this book

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Editors and Affiliations

  • Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

    Giselbert Hauptmann

Bibliographic Information

  • Book Title: In Situ Hybridization Methods

  • Editors: Giselbert Hauptmann

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-2303-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2302-1Published: 19 February 2015

  • Softcover ISBN: 978-1-4939-4463-7Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2303-8Published: 18 February 2015

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XX, 592

  • Number of Illustrations: 15 b/w illustrations, 94 illustrations in colour

  • Topics: Neurosciences, Neurobiology

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