- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproductible results
- Contains key notes and implementation advice from the experts
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- About this book
-
This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.
- Table of contents (26 chapters)
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Key Points to Consider When Studying RNA Remodeling by Proteins
Pages 1-16
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Happy Birthday: 25 Years of DEAD-Box Proteins
Pages 17-33
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In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins
Pages 35-47
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Dynamics of the Spb4 Interactome Monitored by Affinity Purification
Pages 49-67
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Using EMOTE to Map the Exact 5′-Ends of Processed RNA on a Transcriptome-Wide Scale
Pages 69-85
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Table of contents (26 chapters)
- Download Preface 1 PDF (95.6 KB)
- Download Sample pages 1 PDF (556.5 KB)
- Download Table of contents PDF (97.8 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- RNA Remodeling Proteins
- Book Subtitle
- Methods and Protocols
- Editors
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- Marc Boudvillain
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1259
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2214-7
- DOI
- 10.1007/978-1-4939-2214-7
- Hardcover ISBN
- 978-1-4939-2213-0
- Softcover ISBN
- 978-1-4939-4899-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 470
- Number of Illustrations
- 44 b/w illustrations, 40 illustrations in colour
- Topics
