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Methods in Molecular Biology

Bacterial Artificial Chromosomes

Editors: Narayanan, Kumaran (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 91,62 €
price for Spain (gross)
  • ISBN 978-1-4939-1652-8
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Hardcover 124,79 €
price for Spain (gross)
  • ISBN 978-1-4939-1651-1
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  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 114,39 €
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  • ISBN 978-1-4939-5430-8
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  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
About this book

Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.

Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • From Selective Full-Length Genes Isolation by TAR Cloning in Yeast to Their Expression from HAC Vectors in Human Cells

    Pages 3-26

    Kouprina, Natalay (et al.)

  • Recombineering Linear BACs

    Pages 27-54

    Chen, Qingwen (et al.)

  • BAC Sequencing Using Pooled Methods

    Pages 55-67

    Saski, Christopher A. (et al.)

  • Making BAC Transgene Constructs with Lambda-Red Recombineering System for Transgenic Animals or Cell Lines

    Pages 71-98

    Holmes, Scott (et al.)

  • Directing Enhancer-Traps and iTol2 End-Sequences to Deleted BAC Ends with loxP- and lox511-Tn10 Transposons

    Pages 99-122

    Chatterjee, Pradeep K.

Buy this book

eBook 91,62 €
price for Spain (gross)
  • ISBN 978-1-4939-1652-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 124,79 €
price for Spain (gross)
  • ISBN 978-1-4939-1651-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 114,39 €
price for Spain (gross)
  • ISBN 978-1-4939-5430-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Artificial Chromosomes
Editors
  • Kumaran Narayanan
Series Title
Methods in Molecular Biology
Series Volume
1227
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1652-8
DOI
10.1007/978-1-4939-1652-8
Hardcover ISBN
978-1-4939-1651-1
Softcover ISBN
978-1-4939-5430-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 348
Number of Illustrations
12 b/w illustrations, 36 illustrations in colour
Topics