Methods in Molecular Biology

Cytotoxic T-Cells

Methods and Protocols

Editors: Ranieri, Elena (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 93,08 €
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  • ISBN 978-1-4939-1158-5
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Hardcover 176,79 €
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Softcover 114,39 €
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About this book

​ In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8+ T Cells from Blood and Tumor Tissue

    Pages 1-11

    Gigante, Margherita, Ph.D. (et al.)

  • In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes

    Pages 13-20

    Gigante, Margherita, Ph.D. (et al.)

  • Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry

    Pages 21-47

    Popescu, Iulia, Ph.D. (et al.)

  • Intracellular Signaling of CTLs

    Pages 49-63

    Cox, Sharon Natasha, Ph.D. MS

  • Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR

    Pages 65-74

    Lowe, Devin B., Ph.D. (et al.)

Buy this book

eBook 93,08 €
price for Spain (gross)
  • ISBN 978-1-4939-1158-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 176,79 €
price for Spain (gross)
Softcover 114,39 €
price for Spain (gross)
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Bibliographic Information

Bibliographic Information
Book Title
Cytotoxic T-Cells
Book Subtitle
Methods and Protocols
Editors
  • Elena Ranieri
Series Title
Methods in Molecular Biology
Series Volume
1186
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-1158-5
DOI
10.1007/978-1-4939-1158-5
Hardcover ISBN
978-1-4939-1157-8
Softcover ISBN
978-1-4939-4686-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 301
Number of Illustrations
6 b/w illustrations, 26 illustrations in colour
Topics