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Plant MAP Kinases

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. George Komis

    1. Palacky University in Olomouc, Olomouc, Czech Republic

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    You can also search for this editor in PubMed   Google Scholar

  2. Jozef Šamaj

    1. Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1171)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. Expression and Activity of Plant MAPKs

    1. Front Matter

      Pages 1-1
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    2. Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-Based Functional Analysis of MAPK Cascades Signaling

      • Jong Hee Im, Sang-Dong Yoo
      Pages 3-12

    3. Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

      • Pavel Křenek, Veronika Smékalová
      Pages 13-25

    4. Analysis of MAPK Activities Using MAPK-Specific Antibodies

      • Roland Willmann, Daniel J. Haischer, Andrea A. Gust
      Pages 27-37

    5. Detection of Protein Phosphorylation and Charge Isoforms Using Vertical One-Dimensional Isoelectric Focusing Gels

      • Jeffrey C. Anderson, Scott C. Peck
      Pages 39-46

    6. Affinity-Based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™

      • George Komis, Tomáš Takáč, Slávka Bekešová, Pavol Vadovič, Jozef Šamaj
      Pages 47-63

  3. Genetic Manipulation of MAPK Function

    1. Front Matter

      Pages 65-65
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    2. Identification of Constitutively Active AtMPK6 Mutants Using a Functional Screen in Saccharomyces cerevisiae

      • Elodie Hudik, Souha Berriri, Heribert Hirt, Jean Colcombet
      Pages 67-77

    3. Virus-Induced Gene Silencing in Plant MAPK Research

      • Christian Hettenhausen, Ian T. Baldwin, Jianqiang Wu
      Pages 79-89

    4. RNA Interference of Plant MAPK Cascades for Functional Studies

      • Juan Xu, Shuqun Zhang
      Pages 91-103

  4. Localization Studies of MAPKs and Their Interactions

    1. Front Matter

      Pages 105-105
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    2. Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole-Mount Probes

      • Olga Šamajová, George Komis, Jozef Šamaj
      Pages 107-115

    3. Immunofluorescent Localization of MAPKs in Steedman’s Wax Sections

      • Miroslav Ovečka, Olga Šamajová, František Baluška, Jozef Šamaj
      Pages 117-130

    4. Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies

      • Anna Doskočilová, Ivan Luptovčiak, Veronika Smékalová, Jozef Šamaj
      Pages 131-145

    5. Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases

      • Alois Schweighofer, Volodymyr Shubchynskyy, Vaiva Kazanaviciute, Armin Djamei, Irute Meskiene
      Pages 147-158

  5. Analysis of MAPK Substrates

    1. Front Matter

      Pages 159-159
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    2. Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1

      • Andrei Smertenko
      Pages 161-170

    3. In Vivo Phosphorylation of WRKY Transcription Factor by MAPK

      • Nobuaki Ishihama, Hiroaki Adachi, Miki Yoshioka, Hirofumi Yoshioka
      Pages 171-181

    4. Rapid Mutagenesis-Based Analysis of Phosphorylation Sites in Mitogen-Activated Protein Kinase Substrates

      • Lennart Eschen-Lippold, Nicole Bauer, Julia Löhr, Mieder A. T. Palm-Forster, Justin Lee
      Pages 183-192
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Keywords

About this book

Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.

Editors and Affiliations

  • Palacky University in Olomouc, Olomouc, Czech Republic

    George Komis

  • Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic

    Jozef Šamaj

Bibliographic Information

  • Book Title: Plant MAP Kinases

  • Book Subtitle: Methods and Protocols

  • Editors: George Komis, Jozef Šamaj

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0922-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0921-6Published: 07 June 2014

  • Softcover ISBN: 978-1-4939-4589-4Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0922-3Published: 07 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 266

  • Number of Illustrations: 16 b/w illustrations, 17 illustrations in colour

  • Topics: Plant Sciences

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