Methods in Molecular Biology

Stem Cell Transcriptional Networks

Methods and Protocols

Editors: Kidder, Benjamin L (Ed.)

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  • Includes cutting-edge methods and protocols for the interrogation of stem cell transcriptional networks
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-0512-6
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Hardcover 166,39 €
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  • ISBN 978-1-4939-0511-9
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Softcover 109,19 €
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  • ISBN 978-1-4939-5406-3
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About this book

Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Efficient Library Preparation for Next-Generation Sequencing Analysis of Genome-Wide Epigenetic and Transcriptional Landscapes in Embryonic Stem Cells

    Pages 3-20

    Kidder, Benjamin L., Ph.D. (et al.)

  • Analysis of Next-Generation Sequencing Data Using Galaxy

    Pages 21-43

    Blankenberg, Daniel (et al.)

  • edgeR for Differential RNA-seq and ChIP-seq Analysis: An Application to Stem Cell Biology

    Pages 45-79

    Nikolayeva, Olga (et al.)

  • Use Model-Based Analysis of ChIP-Seq (MACS) to Analyze Short Reads Generated by Sequencing Protein–DNA Interactions in Embryonic Stem Cells

    Pages 81-95

    Liu, Tao

  • Spatial Clustering for Identification of ChIP-Enriched Regions (SICER) to Map Regions of Histone Methylation Patterns in Embryonic Stem Cells

    Pages 97-111

    Xu, Shiliyang (et al.)

Buy this book

eBook 88,80 €
price for Spain (gross)
  • ISBN 978-1-4939-0512-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 166,39 €
price for Spain (gross)
  • ISBN 978-1-4939-0511-9
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
Softcover 109,19 €
price for Spain (gross)
  • ISBN 978-1-4939-5406-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
Stem Cell Transcriptional Networks
Book Subtitle
Methods and Protocols
Editors
  • Benjamin L Kidder
Series Title
Methods in Molecular Biology
Series Volume
1150
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-0512-6
DOI
10.1007/978-1-4939-0512-6
Hardcover ISBN
978-1-4939-0511-9
Softcover ISBN
978-1-4939-5406-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 286
Number of Illustrations
8 b/w illustrations, 142 illustrations in colour
Topics