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Microbial Biofilms

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Gianfranco Donelli

    1. Fondazione Santa Lucia IRCCS Microbial Biofilm Laboratory, Rome, Italy

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1147)

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About this book

The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms.

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Keywords

Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Investigations on Biofilms in Health and Disease

    1. Front Matter

      Pages 1-1
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    2. Methods for Dynamic Investigations of Surface-Attached In Vitro Bacterial and Fungal Biofilms

      • Claus Sternberg, Thomas Bjarnsholt, Mark Shirtliff
      Pages 3-22

    3. Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms

      • Mohammed Dwidar, Shuichi Takayama, Robert J. Mitchell
      Pages 23-32

    4. Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and Enterococcal fsr Systems

      • Akane Shojima, Jiro Nakayama
      Pages 33-41

    5. Advanced Techniques for In Situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy

      • Thomas R. Neu, John R. Lawrence
      Pages 43-64

    6. Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyze Multispecies Oral Biofilms

      • Lamprini Karygianni, Elmar Hellwig, Ali Al-Ahmad
      Pages 65-72

    7. Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections

      • Claudia Vuotto, Gianfranco Donelli
      Pages 73-84

    8. Experimental Approaches to Investigating the Vaginal Biofilm Microbiome

      • Marc M. Baum, Manjula Gunawardana, Paul Webster
      Pages 85-103

    9. Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopedic Infections

      • Laura Nistico, Luanne Hall-Stoodley, Paul Stoodley
      Pages 105-126

    10. Animal Models to Evaluate Bacterial Biofilm Development

      • Kim Thomsen, Hannah Trøstrup, Claus Moser
      Pages 127-139

    11. Animal Models to Investigate Fungal Biofilm Formation

      • Jyotsna Chandra, Eric Pearlman, Mahmoud A. Ghannoum
      Pages 141-157

    12. Nonmammalian Model Systems to Investigate Fungal Biofilms

      • Marios Arvanitis, Beth Burgwyn Fuchs, Eleftherios Mylonakis
      Pages 159-172

  3. Investigations on Anti-biofilm Compounds and Strategies

    1. Front Matter

      Pages 173-173
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    2. Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors

      • Livia Leoni, Paolo Landini
      Pages 175-186

    3. In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development

      • Marion Girardot, Christine Imbert
      Pages 187-201

    4. Biofilm Matrix-Degrading Enzymes

      • Jeffrey B. Kaplan
      Pages 203-213

    5. Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices

      • Iolanda Francolini, Antonella Piozzi, Gianfranco Donelli
      Pages 215-225

    6. Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy

      • Wean Sin Cheow, Kunn Hadinoto
      Pages 227-238

    7. Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa In Vitro and In Vivo

      • Wang Hengzhuang, Niels Høiby, Oana Ciofu
      Pages 239-254
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Reviews

From the book reviews:

“This book … is organized in three parts: Investigations on biofilms in health and disease, Investigations on anti-biofilm compounds and strategies, and Investigations on biofilms in the environment and manufacturing plants. … this book should be read by students, researchers, and teachers working in the field of biofilms, as well as scientists working in Microbiology, Ecology or Medicine.” (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)

Editors and Affiliations

  • Fondazione Santa Lucia IRCCS Microbial Biofilm Laboratory, Rome, Italy

    Gianfranco Donelli

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Bibliographic Information

  • Book Title: Microbial Biofilms

  • Book Subtitle: Methods and Protocols

  • Editors: Gianfranco Donelli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0467-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0466-2Published: 25 March 2014

  • Softcover ISBN: 978-1-4939-4676-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0467-9Published: 24 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 380

  • Number of Illustrations: 38 b/w illustrations, 62 illustrations in colour

  • Topics: Microbiology, Bacteriology

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