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In Situ Hybridization in Brain

Editors: Uhl, George (Ed.)

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About this book

The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Cellular Location and Regulation of Proenkephalin mRNA in Rat Brain

    Pages 3-20

    Shivers, Brenda D. (et al.)

  • Vasopressin and Somatostatin mRNA In Situ Hybridization

    Pages 21-47

    Uhl, George R. (et al.)

  • Opioid Peptides and Vasopressin

    Pages 49-62

    Sherman, Thomas G. (et al.)

  • Detection of Neuropeptide mRNAs by In Situ Hybridization Histochemistry

    Pages 63-71

    Siegel, Ruth E. (et al.)

  • In Situ Hybridization as a Quantitative Autoradiographic Method: Vasopressin and Oxytocin Gene Transcription in the Brattleboro Rat

    Pages 73-95

    McCabe, Joseph T. (et al.)

Buy this book

eBook 71,68 €
price for Spain (gross)
  • ISBN 978-1-4615-9486-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover 90,47 €
price for Spain (gross)
  • ISBN 978-1-4615-9488-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
  • The final prices may differ from the prices shown due to specifics of VAT rules
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Bibliographic Information

Bibliographic Information
Book Title
In Situ Hybridization in Brain
Editors
  • George Uhl
Copyright
1986
Publisher
Springer US
Copyright Holder
Plenum Press, New York
eBook ISBN
978-1-4615-9486-4
DOI
10.1007/978-1-4615-9486-4
Softcover ISBN
978-1-4615-9488-8
Edition Number
1
Number of Pages
XX, 300
Topics

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