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Biophysical Chemistry of Proteins

An Introduction to Laboratory Methods

  • Textbook
  • © 2011

Overview

Authors:
  1. Engelbert Buxbaum

    1. P.O. Box 266, Biochemistry, Ross University School of Medicine, Roseau, Dominica

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  • Focuses on the biophysical chemistry of proteins

  • Presents an overview of the methods used in protein research

  • Examines the possible applications and limitations of protein research

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Table of contents (53 chapters)

  1. Front Matter

    Pages i-xvi
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  2. Analytical Techniques

    1. Front Matter

      Pages 1-1
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    2. Microscopy

      • Engelbert Buxbaum
      Pages 3-22

    3. Single Molecule Techniques

      • Engelbert Buxbaum
      Pages 23-24

    4. Preparation of Cells and Tissues for Microscopy

      • Engelbert Buxbaum
      Pages 25-26

    5. Principles of Optical Spectroscopy

      • Engelbert Buxbaum
      Pages 27-31

    6. Photometry

      • Engelbert Buxbaum
      Pages 33-37

    7. Fluorimetry

      • Engelbert Buxbaum
      Pages 39-55

    8. Chemiluminescence

      • Engelbert Buxbaum
      Pages 57-60

    9. Electrophoresis

      • Engelbert Buxbaum
      Pages 61-95

    10. Immunological Methods

      • Engelbert Buxbaum
      Pages 97-122

    11. Isotope Techniques

      • Engelbert Buxbaum
      Pages 123-132

  3. Purification of Proteins

    1. Front Matter

      Pages 133-133
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    2. Homogenisation and Fractionisation of Cells and Tissues

      • Engelbert Buxbaum
      Pages 135-139

    3. Isolation of Organelles

      • Engelbert Buxbaum
      Pages 141-141

    4. Precipitation Methods

      • Engelbert Buxbaum
      Pages 143-146

    5. Chromatography

      • Engelbert Buxbaum
      Pages 147-162

    6. Membrane Proteins

      • Engelbert Buxbaum
      Pages 165-182

    7. Determination of Protein Concentration

      • Engelbert Buxbaum
      Pages 183-186

    8. Cell Culture

      • Engelbert Buxbaum
      Pages 187-189
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About this book

The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

Authors and Affiliations

  • P.O. Box 266, Biochemistry, Ross University School of Medicine, Roseau, Dominica

    Engelbert Buxbaum

About the author

Dr. Buxbaum's research interests are enzymology and protein structure/function relationship. In addition, he has been teaching science and medical students in several countries. He is currently working as associate professor of biochemistry at Ross University School of Medicine.

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