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  • Conference proceedings
  • © 2005

From Cells to Proteins: Imaging Nature across Dimensions

Proceedings of the NATO Advanced Study Institute, held in Pisa, Italy, 12-23 September 2004

  • Updated review of all kinds of microscopy
  • Clear and exhaustive presentation of wide spread biological applications
  • Updated review of hardware and software components in microscopy instrumentation
  • Includes supplementary material: sn.pub/extras

Part of the book series: Nato Security through Science Series B: (NASTB)

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Table of contents (25 papers)

  1. Front Matter

    Pages i-xii
  2. Optical Microscopy for Cell Imaging

    • John M. Girkin
    Pages 1-22
  3. Introduction to Transmission and Scanning Electron Microscopy

    • Franco Verni, Simone Gabrielli
    Pages 23-35
  4. Potential and Limitation of Cytochemistry

    • Giovanna Rosati, Simone Gabrielli
    Pages 37-52
  5. Inside the Small Length and Energy Scales of the World of the Individual Biological Molecule

    • Massimo Sandal, Giampaolo Zuccheri, Bruno Samorì
    Pages 111-137
  6. Scanning Probe Microscopy across Dimensions

    • Ille C. Gebeshuber, Richard A. P. Smith, Hannspeter Winter, Friedrich Aumayr
    Pages 139-165
  7. Imaging Cells Using Soft X-Rays

    • Tom W. Ford
    Pages 167-185
  8. From Microscopy to Nanoscopy: How to Get and Read Optical Data at Single Molecule Level Using Confocal and Two-Photon Excitation Microscopy

    • Alberto Diaspro, Paolo Bianchini, Valentina Caorsi, Davide Mazza, Mattia Pesce, Ilaria Testa et al.
    Pages 187-207
  9. Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM)

    • Thomas M. Jovin, Diane S. Lidke, Elizabeth A. Jares-Erijman
    Pages 209-216
  10. Quantum Dots, a New Tool for Real-Time in Vivo Imaging

    • Donna J. Arndt-Jovin, Thomas M. Jovin, Diane S. Lidke
    Pages 217-225
  11. Wavelength as the Fourth Dimension in Light Microscopy

    • Valtere Evangelista, Laura Barsanti, Anna Maria Frassanito, Vincenzo Passarelli, Paolo Gualtieri
    Pages 323-333
  12. Time as the Fifth Dimension in Microscopy

    • Primo Coltelli, Laura Barsanti, Valtere Evangelista, Anna Maria Frassanito, Paolo Gualtieri
    Pages 335-348
  13. Image Deconvolution

    • M. Bertero, P. Boccacci
    Pages 349-370
  14. Image Formation in Fluorescence Microscopy

    • Giuseppe Vicidomini
    Pages 371-393
  15. Atomic Force Microscopy Study on the Pellicle of the Alga Euglena gracilis

    • Francesca Sbrana, Laura Barsanti, Vincenzo Passarelli, Paolo Gualtieri
    Pages 395-403

About this book

How deep we can see inside Nature's smallest secrets? Will it be possible some day in the near future to investigate living structures at atomic level? This area of study is very interdisciplinary, since it applies the principles and the techniques of biology, physics, chemistry, mathematics, and engineering to elucidate the structures of biological macromolecules, of supramolecular structures, organelles, and cells. This book offers updated information on how much information we are able to obtain in the exploration of the inner details of biological specimens in their native structure and composition.

The book deals with the implementation of laser beam and stage scanning systems incorporating confocal optics or multiphoton microscopy; the advent of new electro-optical detectors with great sensitivity, linearity, and dynamic range; the possibility of 2D fast image enhancement, reconstruction, restoration, analysis and 3D display, and the application of luminescence techniques (FLIMT, FRET combined with the use of quantum dots), which gives the possibility to investigate the chemical and molecular spatio-temporal organization of life processes; Electron Microscopy and Scanning Force Microscopy (SFM), are also presented, which has opened completely new perspectives for analyzing the surface topography of biological matter in its aqueous environment at a resolution comparable to that achieved by EM.

Editors and Affiliations

  • CNR Institute of Biophysics, Pisa, Italy

    Valtere Evangelista, Laura Barsanti, Vincenzo Passarelli, Paolo Gualtieri

Bibliographic Information

Buy it now

Buying options

eBook USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access